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Competitive electrochemical immunosensor for maduramicin detection by multiple signal amplification strategy via hemin@Fe-MIL-88NH(2)/AuPt

文献类型： 外文期刊

作者： Hu, Mei 1 ; Wang, Yao 3 ; Yang, Jifei 2 ; Sun, Yaning 2 ; Xing, Guangxu 2 ; Deng, Ruiguang 2 ; Hu, Xiaofei 2 ; Zhang, Gai 1 ;

作者机构： 1.Jiangnan Univ, Sch Food Sci & Technol, Wuxi 214122, Jiangsu, Peoples R China

2.Henan Acad Agr Sci, Key Lab Anim Immunol, Minist Agr, Zhengzhou 450002, Henan, Peoples R China

3.Henan Univ Sci & Technol, Coll Food & Bioengn, Luoyang 471023, Peoples R China

4.Henan Agr Univ, Zhengzhou 450002, Henan, Peoples R China

关键词： Maduramicin; Metal-organic frameworks; Hemin; Horseradish peroxidase; Hydrogen peroxide; Electrochemical immunosensor

期刊名称：BIOSENSORS & BIOELECTRONICS （ 影响因子：10.618； 五年影响因子：9.323 ）

ISSN： 0956-5663

年卷期： 2019 年 142 卷

页码：

收录情况： SCI

摘要： Maduramicin (MD) is a type of monoglycoside polyether ionophore antibiotic that can effectively treat coccidiosis and facilitate animal growth. However, its extensive and excessive use brings potential risk to human health. Herein, an electrochemical immunosensor based on indirect competitive format was fabricated for analysis of MD residue in eggs by a multiple signal amplification system. Initially, Au nanoparticles were deposited onto glassy carbon electrode surface to load the coating antigen MD-BSA and to improve conductivity. Then the signal amplification platform was constructed by encapsulating hemin into Fe-MIL-88 NH2 metal-organic frameworks (hemin@MOFs), and then the obtained composites were decorated with AuPt nanoparticles. The synthesized hemin@MOFs/AuPt was not only used as a signal amplification mediator, but also utilized as a carrier for immobilization of horseradish peroxidase-conjugated affinipure goat anti-mouse antibody (Ab(2)-HRP) and horseradish peroxidase (HRP). The constructed hemin@MOFs/AuPt-Ab(2)-HRP bioconjugates could effectively amplify the current signal since hemin@MOFs, AuPt and HRP all exhibited high catalytic activity towards the hydrogen peroxide. Moreover, the established immunosensor showed high sensitivity and stability during the detection procedure. With the synergistic catalytic effect of hemin@MOFs, AuPt and HRP, a wide detection range of 0.1-50 ng mL(-1) and a low detection limit of 0.045 ng mL(-1) were achieved (S/N = 3), respectively. Ultimately, the developed method displayed excellent performance in practical applications, providing a promising probability to detect other veterinary drug residues to guarantee food safety.

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