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Combined QTL-Seq and Traditional Linkage Analysis to Identify Candidate Genes for Purple Skin of Radish Fleshy Taproots

文献类型： 外文期刊

作者： Liu, Tongjin 1 ; Wang, Jinglei 1 ; Wu, Chunhui 1 ; Zhang, Youjun 1 ; Zhang, Xiaohui 1 ; Li, Xiaoman 1 ; Wang, Haiping; 1 ;

作者机构： 1.Chinese Acad Agr Sci, Key Lab Biol & Genet Improvement Hort Crops, Minist Agr, Inst Vegetables & Flowers, Beijing, Peoples R China

2.Zhejiang Acad Agr Sci, Inst Vegetables Res, Hangzhou, Zhejiang, Peoples R China

关键词： radish; purple taproot skin; inheritance; OTL-seq; candidate gene

期刊名称：FRONTIERS IN GENETICS （ 影响因子：4.599； 五年影响因子：4.888 ）

ISSN：

年卷期： 2019 年 10 卷

页码：

收录情况： SCI

摘要： Taproot skin color is a crucial visual and nutritional quality trait of radish, and purple skin is most attractive to consumers. However, the genetic mechanism underlying this character is unknown. Herein, F-2 segregating populations were constructed to investigate radish genomic regions with purple skin genes. Segregation analysis suggested that pigment presence was controlled by one dominant gene, Rsps. A bulk segregant approach coupled to whole-genome sequencing (QTL-seq) and classical linkage mapping narrowed the Rsps location to a 238.51-kb region containing 18 genes. A gene in this region, designated RsMYB1 .1 (an Arabidopsis PAP1 homolog), was a likely candidate gene because semiquantitative RT-PCR and quantitative real-time PCR revealed RsMYB1 .1 expression in only purple-skinned genotypes, sequence variation was found between white- and purple-skinned radishes, and an InDel marker in this gene correctly predicted taproot skin color. Furthermore, four RsMYB1 .1 homologs (RsMYB1 .1 -1 .4) were found in "XYB36-2" radish. RsMYB1 .1 and the previously mapped and cloned RsMYB1 .4 (contributing to red skin) were located on different chromosomes and in different subclades of a phylogenetic tree; thus, they are different genes. These findings provide insight into the complex anthocyanin biosynthesis regulation in radish and information for molecular breeding to improve the anthocyanin content and appearance of radish taproots.

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