Complete genome sequence and characterization of a new iflavirus from the small brown planthopper (Laodelphax striatellus)
文献类型： 外文期刊
作者： Wu, Nan 1 ; Zhang, Peipei 2 ; Liu, Wenwen 1 ; Cao, Mengji 3 ; Massart, Sebastien 4 ; Wang, Xifeng 1 ;
作者机构： 1.Chinese Acad Agr Sci, Inst Plant Protect, State Key Lab Biol Plant Dis & Insect Pests, Beijing 100193, Peoples R China
2.Langfang Normal Univ, Coll Life Sci, Langfang 065000, Peoples R China
3.Southwest Univ, Natl Citrus Engn Res Ctr, Citrus Res Inst, Chongqing 400712, Peoples R China
4.Univ Liege, Lab Phytopathol, Gembloux AgrobioTech, Passage Deportes 2, B-5030 Gembloux, Belgium
关键词： Laodelphax striatellus; Insect virus; Iflaviridae; RNA-seq; Characterization
期刊名称：VIRUS RESEARCH （ 影响因子：3.303； 五年影响因子：3.445 ）
ISSN： 0168-1702
年卷期： 2019 年 272 卷
页码：
收录情况： SCI
摘要： A novel iflavirus, tentatively named laodelphax striatellus iflavirus 1 (LsIV1), was identified in Laodelphax striatellus by total RNA-sequencing, and its genome sequence was confirmed by Sanger sequencing. The complete genome consisted of 10,831 nucleotides with a polyA tail and included one open reading frame, encoding a 361.7-kD polyprotein. Conserved motifs for structural proteins, helicase, protease, and RNA-dependent RNA polymerase were identified by aligning the deduced amino acid sequence of LsIV1 with several other iflaviruses. The genome has the highest identity with another planthopper iflavirus, nilaparvata lugens honeydew virus-3 (39.7%), under the species demarcation threshold (90%). Results of the identities and phylogenetic analysis based on the deduced amino acid sequences of the complete polyprotein and helicase of LsIV1 and other iflaviruses, indicated it is a new species belonging to the family Ifiaviridae. Furthermore, we did not observe any differences of biological characterizations like development and reproduction between viruliferous and virus-free SBPH. Meanwhile, we found that female could transmit LsIV1 with higher transmission efficiency.
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