First report of megalocytivirus (iridoviridae) in cultured bluegill sunfish, Lepomis macrochirus, in China
文献类型： 外文期刊
作者： Liu, Lihui 1 ; Yu, Lujun 2 ; Fu, Xiaozhe 1 ; Lin, Qiang 1 ; Liang, Hongru 1 ; Niu, Yinjie 1 ; Li, Ningqiu 1 ;
作者机构： 1.Chinese Acad Fishery Sci, Pearl River Fisheries Res Inst, Key Lab Fishery Drug Dev, Minist Agr & Rural Affairs,Key Lab Aquat Anim Imm, Guangzhou 510380, Guangdong, Peoples R China
2.Guangdong Lab Anim Monitoring Inst, Guangdong Prov Key Lab Lab Anim, Guangzhou 510663, Guangdong, Peoples R China
关键词： Bluegill sunfish; Propagation; Histopathology; ISKNV; Megalocytivirus
期刊名称：MICROBIAL PATHOGENESIS （ 影响因子：3.738； 五年影响因子：3.663 ）
ISSN： 0882-4010
年卷期： 2019 年 135 卷
页码：
收录情况： SCI
摘要： The bluegill sunfish, Lepomis macrochirus, is an important aquacultural and recreational species in southern China because of its excellent taste, rapid growth rate, and good looks. At present, few pathogens are known to affect the bluegill sunfish. However, an iridovirus-like disease recently caused heavy losses to the bluegill sunfish aquaculture industry in Guangdong, China. We report that a virus, designated BSMIV-SD-20171020, was isolated from diseased bluegill sunfish in China. The isolate was efficiently propagated in a Chinese perch brain (CPB) cell line. The cytopathic effect was observed, the MCP gene PCR amplified, and the virus observed with electron microscopy. Its viral titer in CPB cells reached 10(4.13) TCID50 mL(-1). The mortality rate was 100% when bluegill sunfish were challenged with BSMIV-SD-20171020 at a dose of 10(3.13) TCID50/fish. A histopathological examination revealed basophilic hypertrophied cells in the intestine, liver, and spleen. A nucleotide sequence alignment and phylogenetic analysis of the major capsid protein revealed that isolate BSMIV-SD-20171020 is the species Infectious spleen and kidney necrosis virus (ISKNV), in the genus Megalocytivirus.
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