Isolation, culture, and identification of duck intestinal epithelial cells and oxidative stress model constructed
文献类型： 外文期刊
作者： Zhang, Hao 1 ; Chen, Fang 1 ; Liang, Zhen-Hua 1 ; Wu, Yan 1 ; Pi, Jin-Song 1 ;
作者机构： 1.Hubei Acad Agr Sci, Inst Anim Husb & Vet Med, Wuhan 430064, Hubei, Peoples R China
关键词： Intestinal epithelial cells; Primary cultivation; Identification; Duck
期刊名称：IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL （ 影响因子：2.416； 五年影响因子：2.117 ）
ISSN： 1071-2690
年卷期： 2019 年 55 卷 9 期
页码：
收录情况： SCI
摘要： Intestinal epithelial cells (IECs) not only have an absorption function but also act as a physical barrier between the body and the intestinal bacterial flora. Damage to IECs leads to the breakdown of this barrier and has negative effects on animal health. Intestinal epithelial damage is frequently associated with long-term acute stress, such as increased temperature and new stress management models. The intestinal epithelial damage caused by environmental stress has been linked to oxidative stress. Until now, the effects of intestinal epithelial antioxidant activity from feed additives and treatments could be tested in ducks only in vivo because of the lack of in vitro cell culture systems. In this study, we describe our protocol for the easy isolation and culture of IECs from the small intestine of duck embryos. Immunofluorescence was used for the cytological identification of IECs. In addition, IEC marker genes (IAP and CDH1) could also be detected in cultured cells. And cell status assessments were performed, and cell proliferation viability was analyzed by CCK-8 assay. Furthermore, we constructed an oxidative stress model to be used to research the oxidative stress response mechanism, and drugs acting on the cell signal transduction pathway. In conclusion, we have developed an effective and rapid protocol for obtaining duck primary IECs and constructed an oxidative stress model. These IECs exhibit features consistent with epithelial cells and could be used to explore the physiological mechanisms of oxidative stress ex vivo.
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