您好，欢迎访问湖北省农业科学院 机构知识库！
湖北省农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

sigma(54)-Dependent regulator DVU2956 switches Desulfovibrio vulgaris from biofilm formation to planktonic growth and regulates hydrogen sulfide production

文献类型： 外文期刊

作者： Zhu, Lei 1 ; Gong, Ting 1 ; Wood, Thammajun L. 1 ; Yamasaki, Ryota 1 ; Wood, Thomas K. 1 ;

作者机构： 1.Penn State Univ, Dept Chem Engn, State Coll, PA 16802 USA

2.Hubei Acad Agr Sci, Hubei Biopesticide Engn Res Ctr, Natl Biopesticide Engn Technol Res Ctr, Biopesticide Branch,Hubei Innovat Ctr Agr Sci & T, Wuhan 430064, Hubei, Peoples R China

期刊名称：ENVIRONMENTAL MICROBIOLOGY （ 影响因子：5.491； 五年影响因子：6.438 ）

ISSN： 1462-2912

年卷期： 2019 年 21 卷 10 期

页码：

收录情况： SCI

摘要： Microbiologically influenced corrosion causes $100 billion in damage per year, and biofilms formed by sulfate-reducing bacteria (SRB) are the major culprit. However, little is known about the regulation of SRB biofilm formation. Using Desulfovibrio vulgaris as a model SRB organism, we compared the transcriptomes of biofilm and planktonic cells and identified that the gene for s54-dependent regulator DVU2956 is repressed in biofilms. Utilizing a novel promoter that is primarily transcribed in biofilms (Pdvu0304), we found production of DVU2956 inhibits biofilm formation by 70%. Corroborating this result, deleting dvu2956 increased biofilm formation, and this biofilm phenotype could be complemented. By producing proteins in biofilms from genes controlled by DVU2956 (dvu2960 and dvu2962), biofilm formation was inhibited almost completely. A second round of RNA-seq for the production of DVU2956 revealed DVU2956 influences electron transport via an Hmc complex (high-molecular-weight cytochrome c encoded by dvu0531-dvu0536) and the Fe-only hydrogenase (encoded by dvu1769, hydA and dvu1770, hydB) to control H2S production. Corroborating these results, producing DVU2956 in biofilms decreased H2S production by half, deleting dvu2956 increased H2S production by 131 +/- 5%, and producing DVU2956 in the dvu2956 strain reduced H(2)Sproduction. Therefore, DVU2956 maintains SRB in the planktonic state and reduces H2S formation.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：湖北省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台