Identification and characterization of a novel light-induced promoter for recombinant protein production in Pleurotus ostreatus
文献类型： 外文期刊
作者： Yin, Chaomin 1 ; Fan, Xiuzhi 1 ; Ma, Kun 1 ; Chen, Zheya 1 ; Shi, Defang 1 ; Yao, Fen 1 ; Gao, Hong 1 ; Ma, Aimin 3 ;
作者机构： 1.Hubei Acad Agr Sci, Inst Agroprod Proc & Nucl Agr Technol, Wuhan 430064, Hubei, Peoples R China
2.Natl Res & Dev Ctr Edible Fungi Proc Wuhan, Wuhan 430064, Hubei, Peoples R China
3.Huazhong Agr Univ, Coll Food Sci & Technol, Wuhan 430070, Hubei, Peoples R China
关键词： Pleurotus ostreatus; lectin; promoter; Agrobacte-rium tumefaciens; light-induction
期刊名称：JOURNAL OF MICROBIOLOGY （ 影响因子：3.422； 五年影响因子：3.28 ）
ISSN： 1225-8873
年卷期： 2020 年 58 卷 1 期
页码：
收录情况： SCI
摘要： A lectin gene (plectin) with a high level of expression was previously identified by comparative transcriptome analysis of Pleurotus ostreatus. In this study, we cloned a 733-bp DNA fragment from the start codon of the plectin gene. Sequence analysis showed that the plectin promoter (Plp) region contained several eukaryotic transcription factor binding motifs, such as the TATA-box, four possible CAAT-box, light responsiveness motifs and MeJA-responsiveness motifs. To determine whether the Plp promoter was a light-regulated promoter, we constructed an expression vector with the fused egfp-hph fragment under the control of the Plp promoter and transformed P. ostreatus mycelia via Agrobacterium tume-faciens. PCR and Southern blot analyses confirmed the Plp-egfp-hph fragment was integrated into the chromosomal DNA of transformants. qRT-PCR, egfp visualization, and intra-cellular egfp determination experiments showed the Plp promoter could be a light-induced promoter that may be suitable for P. ostreatus genetic engineering. This study lays the foundation for gene homologous expression in P. ostreatus.
- 相关文献
作者其他论文 更多>>
-
γ-Irradiation effects on the physicochemical properties and biological activities of Schizophyllum commune polysaccharides
作者：Li, Chen;Hu, Guoyuan;Li, Chen;Zhang, Qi;Yin, Chaomin;Gao, Hong;Fan, Xiuzhi;Shi, Defang;Qiu, Jianhui;Zhang, Qi;Li, Zhenshun;Gao, Hong;Chen, Sheng;Yin, Chaomin
关键词：Polysaccharides; gamma-irradiation; Physicochemical and structure properties; Antioxidant activities
-
Effects of different oil fractions and tannic acid concentrations on konjac glucomannan-stabilized emulsions
作者：Long, Min;Ren, Yuanyuan;Li, Zhenshun;Yin, Chaomin;Sun, Jie
关键词：Konjac glucomannan; Tannic acid; Hydrogen bonds; High internal phase emulsion; Interfacial crosslinking
-
Properties of modified chitosan-based films and coatings and their application in the preservation of edible mushrooms: A review
作者：Zhang, Yingqi;Shi, Defang;Zhang, Yingqi;Huang, Wen;Liu, Ying;Feng, Xi;Ibrahim, Salam A.
关键词：Modified chitosan; Film; Coating; Mushrooms preservation
-
Chemical composition analysis and transcriptomics reveal the R2R3- MYB genes and phenol oxidases regulating the melanin formation in black radish
作者：Zhang, Shuting;Yuan, Guoli;Peng, Zhaoxin;Huang, Yan;Cui, Lei;Xiao, Guilin;Jiao, Zhenbiao;Deng, Xiaohui;Qiu, Zhengming;Yan, Chenghuan;Zhang, Shuting;Peng, Zhaoxin;Cui, Lei;Xiao, Guilin;Jiao, Zhenbiao;Deng, Xiaohui;Qiu, Zhengming;Yan, Chenghuan;Yuan, Guoli;Li, Xiaoyao;Wang, Liping;Yin, Chaomin
关键词：Correlation network; Melanin formation; Phenol oxidases; MYB genes; Raphanus sativus
-
Effect of Different Cooking Methods on the Bioactive Components, Color, Texture, Microstructure, and Volatiles of Shiitake Mushrooms
作者：Yao, Fen;Gao, Hong;Yin, Chao-Min;Shi, De-Fang;Fan, Xiu-Zhi;Shi, De-Fang;Fan, Xiu-Zhi
关键词：shiitake mushroom; bioactive components; color; texture; microstructure; volatiles
-
Transcriptome analysis provides insight into gamma irradiation delaying quality deterioration of postharvest Lentinula edodes during cold storage
作者：Gao, Hong;Fan, Xiuzhi;Yin, Chaomin;Qiao, Yu;Chen, Xueling;Yao, Fen;Shi, Defang;Ye, Shuang;Liu, Yani;Liu, Ying;Liu, Jingyu
关键词：Lentinula edodes; Transcriptome analysis; Gamma irradiation; Quality deterioration
-
Mycelial biomass and intracellular polysaccharides production, characterization, and activities in Auricularia auricula-judae cultured with different carbon sources
作者：Fan, Xiu-Zhi;Yao, Fen;Yin, Chao -Min;Shi, De -Fang;Gao, Hong;Fan, Xiu-Zhi;Shi, De -Fang;Gao, Hong;Gao, Hong
关键词：Auricularia auricula-judae; Submerged culture; Intracellular polysaccharide; Carbon source; Molecular properties; Biological activity
