Identification and characterization of a novel light-induced promoter for recombinant protein production in Pleurotus ostreatus
文献类型： 外文期刊
作者： Yin, Chaomin 1 ; Fan, Xiuzhi 1 ; Ma, Kun 1 ; Chen, Zheya 1 ; Shi, Defang 1 ; Yao, Fen 1 ; Gao, Hong 1 ; Ma, Aimin 3 ;
作者机构： 1.Hubei Acad Agr Sci, Inst Agroprod Proc & Nucl Agr Technol, Wuhan 430064, Hubei, Peoples R China
2.Natl Res & Dev Ctr Edible Fungi Proc Wuhan, Wuhan 430064, Hubei, Peoples R China
3.Huazhong Agr Univ, Coll Food Sci & Technol, Wuhan 430070, Hubei, Peoples R China
关键词： Pleurotus ostreatus; lectin; promoter; Agrobacte-rium tumefaciens; light-induction
期刊名称：JOURNAL OF MICROBIOLOGY （ 影响因子：3.422； 五年影响因子：3.28 ）
ISSN： 1225-8873
年卷期： 2020 年 58 卷 1 期
页码：
收录情况： SCI
摘要： A lectin gene (plectin) with a high level of expression was previously identified by comparative transcriptome analysis of Pleurotus ostreatus. In this study, we cloned a 733-bp DNA fragment from the start codon of the plectin gene. Sequence analysis showed that the plectin promoter (Plp) region contained several eukaryotic transcription factor binding motifs, such as the TATA-box, four possible CAAT-box, light responsiveness motifs and MeJA-responsiveness motifs. To determine whether the Plp promoter was a light-regulated promoter, we constructed an expression vector with the fused egfp-hph fragment under the control of the Plp promoter and transformed P. ostreatus mycelia via Agrobacterium tume-faciens. PCR and Southern blot analyses confirmed the Plp-egfp-hph fragment was integrated into the chromosomal DNA of transformants. qRT-PCR, egfp visualization, and intra-cellular egfp determination experiments showed the Plp promoter could be a light-induced promoter that may be suitable for P. ostreatus genetic engineering. This study lays the foundation for gene homologous expression in P. ostreatus.
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