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miR-181a promotes porcine granulosa cell apoptosis by targeting TGFBR1 via the activin signaling pathway

文献类型： 外文期刊

作者： Zhang, Jia-Qing 1 ; Gao, Bin-Wen 1 ; Guo, Hong-Xia 1 ; Ren, Qiao-Ling 1 ; Wang, Xian-Wei 3 ; Chen, Jun-Feng 1 ; Wang, 1 ;

作者机构： 1.Henan Acad Agr Sci, Inst Anim Husb & Vet Sci, Zhengzhou 450002, Henan, Peoples R China

2.Henan Key Lab Farm Anim Breeding & Nutr Regulat, Zhengzhou 450002, Henan, Peoples R China

3.Henan Prov Anim Husb Gen Stn, Zhengzhou 450008, Henan, Peoples R China

关键词： miR-181a; Porcine granulosa cell; Apoptosis; TGFBR1; TGF-beta signaling

期刊名称：MOLECULAR AND CELLULAR ENDOCRINOLOGY （ 影响因子：4.102； 五年影响因子：4.226 ）

ISSN： 0303-7207

年卷期： 2020 年 499 卷

页码：

收录情况： SCI

摘要： Activin/Smad3 signaling plays a pivotal role in follicle development and atresia. However, the precise mechanisms underlying this process are not yet fully understood. Herein, we identified miR-181a as a central component of activin/Smad3-mediated follicle atresia. miR-181a was strikingly upregulated in porcine atretic follicles, which induced the apoptosis of porcine granulosa cells (GCs) in vitro. Furthermore, the transforming growth factor-beta type 1 receptor (TGFBR1) was confirmed as a direct target of miR-181a by bioinformatics analysis and luciferase assays. Transfection with an miR-181a agomir repressed the TGFBR1 mRNA and protein levels. In addition, TGFBR1 overexpression repressed GC apoptosis, whereas TGFBR1 inhibition promoted GC apoptosis. miR-181a overexpression downregulated the phosphorylation of Smad3 and blocked the activation of TGF-beta signaling. Moreover, activin A downregulated miR-181a expression and upregulated the TGFBR1 and p-Smad3 protein levels. Collectively, these data suggest that miR-181a regulates porcine GC apoptosis by targeting TGFBR1 via the activin signaling pathway.

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