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Morphologic and genomic characterization of a broad host range Salmonella enterica serovar Pullorum lytic phage vB_SPuM_SP116

文献类型： 外文期刊

作者： Bao, Hongduo 1 ; Shahin, Khashayar 1 ; Zhang, Qiaoyan 3 ; Zhang, Hui 1 ; Wang, Zhen 4 ; Zhou, Yan 1 ; Zhang, Xuhui 2 ; Zh 1 ;

作者机构： 1.Jiangsu Acad Agr Sci, IPRC, State Key Lab Breeding Base, Key Lab Food Qual & Safety Jiangsu Prov, Nanjing 210014, Jiangsu, Peoples R China

2.Nanjing Forestry Univ, Coll Forestry, Nanjing 210037, Jiangsu, Peoples R China

3.Zhejiang Acad Agr Sci, Inst Qual & Stand Agroprod, Hangzhou 310021, Zhejiang, Peoples R China

4.Greentown Agr Testing Technol Co Ltd, Hangzhou 310000, Zhejiang, Peoples R China

5.Univ KwaZulu Natal, Coll Agr Engn & Sci, Sch Life Sci, Discipline Microbiol, ZA-3201 Pietermaritzburg, South Africa

关键词： Salmonella enterica serovar Pullorum; Lytic phage; Host range; Felix O1 virus; Myoviridae; Complete genome sequence

期刊名称：MICROBIAL PATHOGENESIS （ 影响因子：3.738； 五年影响因子：3.663 ）

ISSN： 0882-4010

年卷期： 2019 年 136 卷

页码：

收录情况： SCI

摘要： For effective use of phages as antimicrobial agents for controlling multidrug resistant S. Pullorum, it is important to understand phage biology. A lytic S. Pullorum phage was isolated and characterized from chicken feces, and its whole genome was sequenced and analyzed. A new lytic phage-vB_SPuM_SP116 (in brief SP116)- isolated and characterized using S. Pullorum SPu-116 as its host belongs to Myoviridae A1 group. Phage SP116 had a lytic effect on 27 of 37 (72.9%) different serotypes of clinical Salmonella strains. It showed a high bactericidal activity in killing all pathogens in cultures containing 5 x 10(5) cfu/mL and achieved more than 6.58 and 5.97 log unit reductions in cultures containing 5 x 10(6) cfu/mL and 5 x 10(7) cfu/mL, respectively. The one-step growth curve showed that the burst size was up to 118 pfu/bacterial cell. Complete genome sequence analysis revealed a linear, double-stranded DNA genome of 87,510 bp with an average G + C content of 38.84%, including 128 predicted open reading frames (ORFs) and 22 tRNA genes. SP116 was classified as a Felix O1 virus based upon the general phage characterization and the genomic information. Regarding its high efficacy in preventing especially S. Pullorum infection and its lack of any bacterial virulence, antimicrobial resistance, and lysogenesis genes, it could be a potential alternative candidate for the treatment of S. Pullorum infections.

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