Integrated metabolomic and transcriptomic analyses suggest that high dietary lipid levels facilitate ovary development through the enhanced arachidonic acid metabolism, cholesterol biosynthesis and steroid hormone synthesis in Chinese sturgeon (Acipenser sinensis)
文献类型： 外文期刊
作者： Leng, Xiaoqian 1 ; Zhou, Hai 2 ; Tan, Qingsong 2 ; Du, Hao 1 ; Wu, Jinping 1 ; Liang, Xufang 2 ; He, Shan 2 ; Wei, Qiwei 1 ;
作者机构： 1.Chinese Acad Fisheries Sci, Yangtze River Fisheries Res Inst, Minist Agr China, Key Lab Freshwater Biodivers Conservat, Wuhan 430223, Hubei, Peoples R China
2.Huazhong Agr Univ, Fisheries Coll, Hubei Prov Engn Lab Pond Aquaculture,Freshwater A, Key Lab Freshwater Anim Breeding,Minist Agr China, Wuhan 430070, Hubei, Peoples R China
关键词： Chinese sturgeon; Ovary development; Dietary lipid levels; Arachidonic acid metabolism
期刊名称：BRITISH JOURNAL OF NUTRITION （ 影响因子：3.718； 五年影响因子：4.816 ）
ISSN： 0007-1145
年卷期： 2019 年 122 卷 11 期
页码：
收录情况： SCI
摘要： Chinese sturgeon (Acipenser sinensis) is an endangered species, listed as a grade I protected animal in China. The females rarely successfully develop their gonads from stage II to III in captivity, which handicaps the propagation of cultured Chinese sturgeon. The present study aimed to understand the effects of dietary lipid level on the ovarian development and the related regulation mechanism in female Chinese sturgeon. A 24-month feeding trial was conducted with 10-year-old Chinese sturgeons with ovaries at the developmental stage II, with three experimental diets containing 10, 14 and 18 % lipids. Ovary, muscle and serum samples were collected at four time points (6, 12, 18 and 24 months) for further analyses. Serum metabolomics and ovary transcriptomics analyses were conducted at 18 months. Results showed that only the 18 % lipid diet promoted ovary development to stage IV. Oocytes at stage II in this group also exhibited higher diameter and more lipid droplets. Serum TAG content in the 18 % group was significantly higher than in 10 and 14 % groups (both at 12 and 18 months). Oestradiol content in the 14 % group was significantly higher than in 10 and 18 % groups, except at 24 months. Metabolomic and transcriptomic results indirectly indicated that 14 % of dietary lipids benefited steroid hormone synthesis, while 18 % lipid facilitated arachidonic acid metabolism, cholesterol biosynthesis and vitellogenesis, although serum cholesterol content did not vary with dietary lipid level. In conclusion, 18 % dietary lipid is the optimal level for improving gonad development of female Chinese sturgeon.
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