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Molecular cloning and functional analyses of the salt- responsive gene KVHSP70 from Kosteletzkya virginica

文献类型： 外文期刊

作者： Tang, Xiaoli 1 ; Shao, Hongbo 2 ; Jiang, Fudong 5 ; Sheteiwy, Mohamed Salah Amr 2 ; Yang, Ruiping 3 ; Wang, Hongyan 6 ;

作者机构： 1.Ludong Univ, Coll Agr, Yantai 264000, Peoples R China

2.Jiangsu Acad Agr Sci, Key Lab Agr Environm Lower Reaches Yangtze River, Inst Agr Resources & Environm, Salt Soil Agr Ctr, Nanjing 210014, Jiangsu, Peoples R China

3.Yancheng Teachers Univ, Jiangsu Synthet Innovat Ctr Coastal Bioagr, Jiangsu Key Lab Bioresources Saline Soils, Yancheng, Peoples R China

4.Qingdao Univ Sci & Technol, Coll Environm & Safety Engn, Qingdao, Shandong, Peoples R China

5.Yantai Acad Agr Sci, Yantai, Peoples R China

6.Yantai Acad China Agr Univ, Yantai, Peoples R China

7.Ludong Univ, Key Lab Mol Module Based Breeding High Yield & Ab, Yantai, Peoples R China

8.Mansoura Univ, Fac Agr, Dept Agron, Mansoura, Egypt

关键词： K; virginica; KvHSP70; land rehabilitation; salt-resistant breeding; salt-soil improvement and use; salt-stress gene

期刊名称：LAND DEGRADATION & DEVELOPMENT （ 影响因子：4.977； 五年影响因子：5.291 ）

ISSN： 1085-3278

年卷期： 2020 年 31 卷 6 期

页码：

收录情况： SCI

摘要： Land degradation resulting from soil salinization is becoming a growing menace in almost all over the world. The degraded soil shows poor performance in water conductance and soil aeration, which threatens plant growth and development. Therefore, to discover the resistance genes and breed plant with stress tolerance are necessary. The modern molecular biotechnology makes the plant with enhanced adaption possible. To do this, KvHSP70 with 1950 bp open reading frame (ORF) from Kosteletzkya virginica was isolated, studied, and transformed into tobacco for functional study. Our research showed that it encodes a typical HSP70 protein with 649 amino acids and it could be induced by salt, high temperature, drought, and abscisic acid (ABA). The transgenic tobacco with it overexpression had higher chlorophyll content, less electrolyte leakage, lower water potential and increased proline content under salt stress. Meanwhile, the transgenic acquired superior antioxidant enzyme activities and decreased MDA content. All results highlight the significance of the new found gene in breeding salt resistant plants with molecular manipulation. Hence, K. virginica provides us with helpful information and gene resources to culture new variety adapted to salt-affected soil and available for land rehabilitation perhaps in the future.

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