Development of an Indirect ELISA Based on VP1-CRT Fusion Protein for Detection of FMDV-O in Swine
文献类型： 外文期刊
作者： Liu, Chang 1 ; Feng, Hua 2 ; Liu, Yunchao 2 ; Chen, Yumei 3 ; Yang, Suzhen 2 ; Lu, Qingxia 2 ; Feng, Lili 4 ; Deng, Ruigu 1 ;
作者机构： 1.Jilin Univ, Coll Vet Med, Changchun 130033, Jilin, Peoples R China
2.Henan Acad OfAgr Sci, Henan Prov Key Lab Anim Immunol, Minist Agr, Key Lab Anim Immunol, Zhengzhou 450002, Henan, Peoples R China
3.Zhengzhou Univ, Sch Life Sci, Zhengzhou 450001, Henan, Peoples R China
4.Henan Acad Agr Sci, Inst Agr Econ & Informat, Zhengzhou 450002, Peoples R China
5.Henan Agr Univ, Coll Anim Sci & Vet Med, Zhengzhou 450002, Henan, Peoples R China
关键词： FMDV-O VP1; Fusion protein; Polymers; Prokaryotic expression; Indirect ELISA
期刊名称：INTERNATIONAL JOURNAL OF AGRICULTURE AND BIOLOGY （ 影响因子：0.822； 五年影响因子：0.906 ）
ISSN： 1560-8530
年卷期： 2020 年 23 卷 1 期
页码：
收录情况： SCI
摘要： Foot-and-mouth disease (FMD) is a highly contagious viral disease of cloven-hoofed animals The open reading frame (ORF) of VP1 codes the most important structure protein of foot and mouth disease virus (FMDV). To develop a highly sensitive and specific indirect ELISA method for detecting FMDV-O antibody, the prokaryotic optimized gene vp1 was fused with truncated calceticulin (rT5V) and co-expressed with trigger factor (Tf16) which could assist soluble expression of antigenicity polymers in Escherichia coli. The rT5V was purified using size-exclusion chromatography with the purity about 80%. The rT5V-ELISA method was developed using the purified rT5V as coating antigen for FMDV-O antibody detection. The sensitivity and specificity of the rT5V-ELISA were 84.2 and 100%, respectively. The comparative test between rT5V-ELISA and the liquid-phase blocking ELISA kit (LPB-ELISA) with 376 clinical samples showed the coincidence rate was 84.04%. In conclusion, the rT5V was achieved in soluble polymers by co-expressing with Tf16 in E. coli. The rT5V-ELISA was developed as a highly sensitive and specific method for FMDV-O antibody monitoring after infection and/or vaccination. (C) 2020 Friends Science Publishers
- 相关文献
作者其他论文 更多>>
-
Enhancing humoral and mucosal immune response of PED vaccine candidate by fusing S1 protein to nanoparticle multimerization
作者：Li, Minghui;Sun, Xueke;Wang, Siqiao;Wang, Yanan;Wang, Yue;Zhang, Gaiping;Li, Minghui;Sun, Xueke;Chen, Yilan;Wang, Siqiao;Li, Qin;Wang, Yanan;Wang, Yue;Li, Ruiqi;Zhang, Gaiping;Ding, Peiyang;Zhang, Gaiping;Ding, Peiyang;Zhang, Gaiping;Zhang, Gaiping;Zhang, Gaiping
关键词：PEDV; S1 protein; Nanoparticle; Subunit vaccine; Mucosal immunity
-
Pseudorabies virus tegument protein US2 antagonizes antiviral innate immunity by targeting cGAS-STING signaling pathway
作者：Kong, Zhengjie;Zhang, Yifeng;Guan, Kaifeng;Yao, Wanzi;Kang, Yu;Lu, Xinyi;Zhang, Gaiping;Chen, Xing;Gong, Lele;Wang, Lele;Zhang, Yuhang;Du, Yongkun;Sun, Aijun;Zhuang, Guoqing;Wan, Bo;Zhang, Gaiping;Zhao, Jianguo;Zhang, Gaiping;Zhang, Gaiping
关键词：pseudorabies virus; cGAS-STING; tegument protein US2; TRIM21; immune invasion
-
IFI16 Positively Regulates RIG-I-Mediated Type I Interferon Production in a STING-Independent Manner
作者：Shi, Xibao;Wei, Menglu;Feng, Yuwen;Yang, Yuanhao;Zhang, Xiaozhuan;Chen, Hao;Xing, Yuqi;Wang, Keqi;Wang, Wensheng;Wang, Li;Zhang, Gaiping;Wang, Aiping;Zhang, Gaiping;Zhang, Gaiping;Zhang, Gaiping;Shi, Xibao;Zhang, Gaiping
关键词：IFN-gamma-inducible protein 16; interferon gene-stimulating protein; retinoic acid-inducible gene I; type I interferon
-
Characterization of Fusarium solani Associated with Tobacco (Nicotiana tabacum) Root Rot in Henan, China
作者：Qiu, Rui;Li, Caihong;Zhang, Yingying;Li, Xiaojie;Li, Chengjun;Liu, Chang;Zhang, Mengdan;Bai, Jingke;Chen, Yuguo;Li, Shujun;Li, Fangfang
关键词：cross-pathogenicity; genetic diversity; molecular analysis; morphological characteristics; Nectria haematococca
-
A universal design of restructured dimer antigens: Development of a superior vaccine against the paramyxovirus in transgenic rice
作者：Ma, Fanshu;Zhang, Longxian;Zhang, Erqin;Zhang, Gaiping;Ma, Fanshu;Zhang, Gaiping;Ma, Fanshu;Zhang, Gaiping;Ma, Fanshu;Zhang, Gaiping;Ma, Fanshu;Xu, Qianru;Yang, Daichang;Li, Qingmei;Guo, Junqing;Li, Rui;Wang, Li;Wang, Yanan;Zhao, Xiangyue;Chai, Shujun;Yin, Heng;Li, Kunpeng;Rao, Zihe;Rao, Zihe;Rao, Zihe
关键词：structural vaccine; transgenic rice; BCR; paramyxovirus
-
Double-layered N-S1 protein nanoparticle immunization elicits robust cellular immune and broad antibody responses against SARS-CoV-2
作者：Li, Ruiqi;Chang, Zejie;Cheng, Anchun;Zhang, Gaiping;Li, Ruiqi;Zhang, Gaiping;Li, Ruiqi;Zhang, Gaiping;Li, Ruiqi;Chang, Zejie;Wang, Yanan;Li, Minghui;Chen, Yilan;Fan, Lu;Wang, Siqiao;Sun, Xueke;Liu, Siyuan;Zhang, Gaiping;Chang, Zejie;Wang, Yanan;Li, Minghui;Sun, Xueke;Liu, Siyuan;Zhang, Gaiping;Liu, Hongliang;Ding, Peiyang;Zhang, Gaiping
关键词：COVID-19; Coronavirus; SARS-CoV-2; Nanoparticle; Subunit vaccine; Variants
-
Porcine reproductive and respiratory syndrome virus triggers Golgi apparatus fragmentation-mediated autophagy to facilitate viral self-replication
作者：Zhao, Shuang-shuang;Qian, Qisheng;Zhang, Gaiping;Zhao, Shuang-shuang;Qian, Qisheng;Chen, Xin-xin;Lu, Qingxia;Xing, Guangxu;Qiao, Songlin;Li, Rui;Zhang, Gaiping;Zhang, Gaiping
关键词：autophagy; GA fragmentation; GRASP65; Nsp2; PRRSV; RAB2; ULK1
