Molecular cloning of Gibel carp (Carassius auratus gibelio) complement component C3 and its expression profile after Cyprinid herpesvirus 2 infection
文献类型： 外文期刊
作者： Fan, Yuding 1 ; Zhang, Xueping 2 ; Zhou, Yong 1 ; Jiang, Nan 1 ; Liu, Wenzhi 1 ; Zeng, Lingbing 1 ;
作者机构： 1.Chinese Acad Fishery Sci, Yangtze River Fisheries Res Inst, Wuhan 430223, Hubei, Peoples R China
2.Beijing Wisdomab Biotechnol Co Ltd, Beijing 100176, Peoples R China
关键词： cloning; complement component C3; Cyprinid herpesvirus 2 (CyHV-2); Gibel carp (Carassius auratus gibelio); immune response
期刊名称：JOURNAL OF VETERINARY MEDICAL SCIENCE （ 影响因子：1.267； 五年影响因子：1.348 ）
ISSN： 0916-7250
年卷期： 2020 年 82 卷 1 期
页码：
收录情况： SCI
摘要： The complement systems play an important role in innate and adaptive immunity. In this study, the complement C3 gene, designated CagC3, was cloned and sequenced from Gibel carp (Carassius auratus gibelio). The expression pattern of CagC3 in different tissues of healthy Gibel carp and after challenge with Cyprinid herpesvirus 2 (CyHV-2) were evaluated using quantitative real-time PCR. The full-length CagC3 cDNA was 5131 bp with an ORF of 4950 bp, encoding a predicted protein of 1649 amino acids. The deduced amino acid sequence showed that CagC3 has conserved domains and residues known to be critical for C3 function. Phylogenetic analysis demonstrated that CagC3 clustered with homologs from common carp and grass carp (Ctenopharyngodon idella). CagC3 is expressed in all examined tissues of healthy Gibel carp, with the highest expression in liver. In vivo, after CyHV-2 challenge, CagC3 transcription was significantly upregulated in liver, spleen and kidney with the peaks at 24 hr, 2 d, and 2 d, respectively. In vitro, CagC3 expression in the Gibel carp brain cell line showed the same pattern as that in vivo after stimulation with CyHV-2 or poly(I:C). However, CagC3 expression was downregulated at 24 hr after induction with lipopolysaccharide (LPS), and then reached the peak at 2 d. These results suggest that CagC3 is involved in the innate immune response of Gibel carp to viral infection.
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