您好，欢迎访问河南省农业科学院 机构知识库！
河南省农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

FpDep1, a component of Rpd3L histone deacetylase complex, is important for vegetative development, ROS accumulation, and pathogenesis in Fusarium pseudograminearum

文献类型： 外文期刊

作者： Zhang, Yinshan 1 ; Wang, Limin 1 ; Liang, Shen 2 ; Zhang, Panpan 1 ; Kang, Ruijiao 1 ; Zhang, Mengjuan 1 ; Wang, Min 1 ;

作者机构： 1.Henan Agr Univ, Collaborat Innovat Ctr Henan Grain Crops, Natl Key Lab Wheat & Maize Crop Sci, Zhengzhou 450002, Peoples R China

2.Henan Acad Agr Sci, Hort Res Inst, Zhengzhou 450009, Henan, Peoples R China

关键词： Wheat; Fusarium pseudograminearum; Fusarium crown rot; FpDEP1; Rpd3L histone deacetylase complex; Pathogenesis

期刊名称：FUNGAL GENETICS AND BIOLOGY （ 影响因子：3.495； 五年影响因子：3.831 ）

ISSN： 1087-1845

年卷期： 2020 年 135 卷

页码：

收录情况： SCI

摘要： Histone deacetylases (HDACs) play essential roles in modulating chromatin structure to provide accessibility to gene regulators. Increasing evidence has linked HADCs to pathogenesis control in the filamentous plant fungi. However, its function remains unclear in Fusarium pseudograminearum, which has led to the emergence of the disease Fusarium crown rot in China. Here we identified the FpDEP1 gene, an orthologue of Saccharomyces cerevisiae DEP1 encoding a component of the Rpd3 histone deacetylase complex in F. pseudograminearum. The gene deletion mutant, Delta Fpdep1, showed significantly retarded growth on PDA plates with reduced aerial hyphae formation. Pathogenicity tests displayed no typical leaf lesions and limited expansion capability of coleoptiles. Histopathological analysis indicated the Delta Fpdep1 deletion mutant differentiated infectious hyphae and triggered massive reactive oxygen species (ROS) accumulation during the early infection stage, resulting in limited expansion to neighbor cells which was concurring with sensitivity to H2O2 and SDS tests in vitro. FM4-64 staining revealed that the Delta Fpdep1 deletion mutant was delayed in endocytosis. The FpDEP1-GFP transgene complemented the mutant phenotypes and the fusion protein co-localized with DAPI staining, indicating that the FpDEP1 gene product is localized to the nucleus in spores and mycelia. Immunoprecipitation coupled with LC-MS/MS and yeast two-hybrid screening identified the Rpd3L-like HDAC complex containing at least FpDep1, FpSds3, FpSin3, FpRpd3, FpRxt3, FpCti6, FpRho23, and FpUme6. These results suggest that FpDep1 is involved in a HDAC complex functioning on fungal development and pathogenesis in F. pseudograminearum.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：河南省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台