Highly sensitive immunochromatographic assay for simultaneous determination of azaperone and azaperol in pork
文献类型： 外文期刊
作者： Chen, Linlin 1 ; Hu, Xiaofei 1 ; Xing, Yunrui 1 ; Sun, Yaning 1 ; Hu, Mei 3 ; Zhang, Gaiping 1 ;
作者机构： 1.Henan Acad Agr Sci, Key Lab Anim Immunol, Minist Agr, Zhengzhou 450002, Peoples R China
2.Jiangnan Univ, Sch Food Sci & Technol, Wuxi 214122, Jiangsu, Peoples R China
3.Henan Agr Univ, Coll Food Sci & Technol, Zhengzhou 450002, Peoples R China
关键词： Rapid detection; Azaperone; Azaperol; Monoclonal antibody; Immunoassay strip
期刊名称：FOOD CHEMISTRY-X （ 影响因子：6.1； 五年影响因子：6.4 ）
ISSN： 2590-1575
年卷期： 2023 年 17 卷
页码：
收录情况： SCI
摘要： In this study, a sensitive immunochromatographic assay (ICA) was developed for simultaneously detecting azaperone (AZN) and its metabolite azaperol (AZL) based on the high-affinity monoclonal antibody (mAb). Herein, the hapten AZL-SA was synthesized by succinic anhydride method, and then the conjugates AZL-SA-OVA and AZL-SA-KLH were prepared by EDC/NHS method. Subsequently, mAb was produced for targets monitoring through two detection modes. In direct ICA (using gold nanoparticles labeled specific antibodies), the visual LOD of AZN was 80 ng/g. For indirect ICA (using gold nanoparticles labeled anti-species antibodies), the visual and instrumental LODs of AZN were 8 and 0.14 ng/g, and AZL were 8 and 0.12 ng/g, respectively. The results indicated that the visual detection limit (LOD) of indirect format was tenfold lower than that of direct format. The established analytical method obtains the results within 15 min and provides a sensitive and simple tool for on-site detection of AZN and AZL.
- 相关文献
作者其他论文 更多>>
-
Potential Pathogenicity and Genetic Characteristics of a Live-Attenuated Classical Swine Fever Virus Vaccine Derivative Variant
作者：Guo, Zhenhua;Xing, Guangxu;Jin, Qianyue;Lu, Qingxia;Zhang, Gaiping;Wang, Leyi;Wang, Leyi;Zhang, Gaiping;Zhang, Gaiping
关键词：
-
An immunochromatographic strip sensor for marbofloxacin residues
作者：Yang, Xingdong;Zhang, Qianqian;Yang, Xingdong;Li, Qingmei;Yang, Jifei;Hu, Xiaofei;Kwee, Sharon
关键词：
-
Proteomic analysis reveals the antiviral effects of baicalin on pseudorabies virus
作者：Niu, Qiaoge;Zhang, Gaiping;Niu, Qiaoge;Zhou, Chuanjie;Li, Rui;Guo, Junqing;Qiao, Songlin;Chen, Xin-xin;Zhang, Gaiping;Zhang, Gaiping
关键词：Baicalin; Pseudorabies virus; ROS; F3; NFU1; CEBPB
-
Application of Surface-Enhanced Raman Spectroscopy Combined with Immunoassay for the Detection of Adrenoceptor Agonists
作者：Wang, Yao;Jing, Yubing;Cao, Jinbo;Sun, Yingying;Guo, Kaitong;Chen, Xiujin;Li, Zhaozhou;Cao, Jinbo;Guo, Kaitong;Hu, Xiaofei;Shi, Qiaoqiao
关键词：adrenoceptor agonists; SERS; immunoassays; substrate materials; Raman reporter molecules
-
Marine peptides as potential anti-aging agents: Preparation, characterization, mechanisms of action, and future perspectives
作者：Yao, Wanzi;Zhang, Yifeng;Zhang, Gaiping;Zhang, Gaiping;Zhang, Gaiping;Zhang, Gaiping;Zhang, Gaiping
关键词：Marine peptides; Anti-aging; Preparation; Mechanisms; Oxidative stress; Signaling pathways
-
Thiolated chitosan encapsulation constituted mucoadhesive nanovaccine confers broad protection against divergent influenza A viruses
作者：Ding, Peiyang;Liu, Hongliang;Zhu, Xifang;Chen, Yumei;Zhou, Jingming;Wang, Aiping;Zhang, Gaiping;Ding, Peiyang;Liu, Hongliang;Zhu, Xifang;Chen, Yumei;Zhou, Jingming;Wang, Aiping;Zhang, Gaiping;Ding, Peiyang;Liu, Hongliang;Zhu, Xifang;Chen, Yumei;Zhou, Jingming;Wang, Aiping;Zhang, Gaiping;Chai, Shujun;Zhang, Gaiping;Zhang, Gaiping
关键词：Mucoadhesive; Thiolated chitosan; Influenza nanovaccine; M2e; Nucleoprotein
-
Identification of the Linear Fc-Binding Site on the Bovine IgG1 Fc Receptor (boFcγRIII) Using Synthetic Peptides
作者：Wang, Ruining;Guo, Junqing;Yang, Jifei;Li, Qingmei;Zhang, Gaiping;Wang, Ruining;Li, Ge;Wang, Xun;Zhang, Gaiping;Zhang, Gaiping
关键词：BoFc gamma RIII; Fc-binding site; bovine IgG1; synthetic peptides
