文献类型： 外文期刊

作者： Ji, C. ¹ ; Deng, G. ³ ; Guevara-Oquendo, V. H. ⁴ ; Zhang, X. ¹ ; Yan, X. ² ; Zhang, H. ³ ; Yu, P. ⁴ ;

作者机构： 1.Tianjin Agr Univ, Coll Anim Sci & Vet Med, 22 Jinjin Rd, Tianjin 300384, Peoples R China

2.Jilin Acad Agr Sci, Branch Acad Anim Sci, Gongzhuling 136100, Jilin, Peoples R China

3.Foshan Univ, Coll Life Sci & Engn, Foshan, Guangdong, Peoples R China

4.Univ Saskatchewan, Dept Anim & Poultry Sci, Coll Agr & Bioresources, 51 Campus Dr, Saskatoon, SK S7N 5A8, Canada

关键词： Vibrational molecular spectroscopy-ATR-FTIR; Univariate and multivariate molecular spectral analyses; Truly absorbed protein supply; Rumen degradation; Intestine digestion; Protein molecular structure and conformation; Model forages

期刊名称：SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY （ 影响因子：4.098； 五年影响因子：3.464 ）

ISSN： 1386-1425

年卷期： 2020 年 228 卷

页码：

收录情况： SCI

摘要： This study aimed to (1) access protein molecular structure profile and metabolic characteristics of model forages [Foreign sourced-origin (coded as: "FSO", n= 7 vs. Chinese sourced-origin alfalfa hay "CSO", n= 5] in ruminant systems; (2) Quantify the relationship between forage protein molecular structures and protein utilization and availability. Advanced non-invasive vibrational molecular spectroscopic technique (ATR-FTIR: Attenuated Total Reflection Fourier Transform Infrared spectroscopy) with chemometrics was applied to reveal forage protein molecular structure. Both univariate and multivariate molecular spectral analyses were applied to study molecular structure features in model forages. The molecular structure study provided the detailed protein structure profiles of Amide I and Amide II areas and height, total Amide I and II area ratios, Amide I to II height ratio as well as Amide I to II area ratio using ATR-FTIR spectroscopy. The results showed FSO and CSO had similar (P > 0.05) protein rumen degradation kinetics. However, FSO had superior quality than CSO in intestinal (IDP) and total digestible protein (TDP) and truly absorbed nutrient supply (P < 0.05). As intestinal digestion of protein, FSO was higher (P < 0.05) in protein digestion in terms of: intestinal digestibility of rumen undegraded protein (dIDP: 47.5 vs. 38.3 %RUP); Intestinal digestible protein (IDP: 17.6 vs. 13.7 %CP). As truly absorbed nutrient supply, FSO contained higher (P < 0.05) truly absorbed rumen synthesized microbial protein, absorbable rumen undegradable feed protein in the small intestine, total truly digested protein in the small intestine, metabolizable protein and Feed Milk Value (FMVDVE: 1.2 vs. 1.1 g/kg DM). The molecular structure-nutrition interactive relationship study showed that protein molecular structure profiles were highly associated to protein rumen degradation kinetics, significantly correlated to protein subfractions, protein intestinal digestion, and truly absorbed nutrient supply in ruminant systems. (c) 2019 Elsevier B.V. All rights reserved.