Isolation, partial characterization, and cloning of an extracellular chitinase from the entomopathogenic fungus Verticillium lecanii
文献类型： 外文期刊
作者： Yu, G. 1 ; Xie, L. Q. 1 ; Li, J. T. 1 ; Sun, X. H. 1 ; Zhang, H. 2 ; Du, Q. 3 ; Li, Q. Y. 3 ; Zhang, S. H. 1 ; Pan, H. Y. 1 ;
作者机构： 1.Jilin Univ, Coll Plant Sci, Changchun 130023, Peoples R China
2.Jilin Agr Univ, Coll Resource & Environm, Changchun, Jilin, Peoples R China
3.Jilin Acad Agr Sci, Changchun, Peoples R China
关键词： Acidic extracellular chitinase;Inhibitory effect;Protein modeling;Verticillium lecanii
期刊名称：GENETICS AND MOLECULAR RESEARCH （ 影响因子：0.764； 五年影响因子：0.912 ）
ISSN： 1676-5680
年卷期： 2015 年 14 卷 1 期
页码：
收录情况： SCI
摘要： The entomopathogenic fungus Verticillium lecanii is a well-known biocontrol agent of fungal phytopathogens, as well as insect pests. A 42-kDa chitinase belonging to family 18 of the glycosyl hydrolases was isolated and partially characterized. Chitinase was purified using successive column chromatography on phenyl-sepharose, DEAE-sepharose, and CM-sepharose. The enzyme showed the highest activity at 40 degrees C and pH 4.6. Enzyme activity was strongly activated in the presence of Mg2+. The purified enzyme showed inhibitory activity of spore germination against several plant pathogens, particularly Fusarium moniliforme. The genomic DNA and cDNA sequences were resolved by polymerase chain reaction amplification and sequencing. Protein modeling and comparative investigation of different chitinase amino acids showed that chitinases are conserved in parasitic fungi.
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