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Immune-related miRNA-mRNA regulation network in the livers of DHAV-3-infected ducklings

文献类型： 外文期刊

作者： Wu, Fengyao 1 ; Lu, Fengying 1 ; Fan, Xin 1 ; Chao, Jin 1 ; Liu, Chuanmin 1 ; Pan, Qunxing 1 ; Sun, Huawei 1 ; Zhang, Xia 1 ;

作者机构： 1.Jiangsu Acad Agr Sci, Inst Vet Med, Nanjing, Jiangsu, Peoples R China

2.Minist Agr, Key Lab Vet Biol Engn & Technol, Nanjing, Jiangsu, Peoples R China

3.Tibet Agr & Anim Husb Univ, Acad Anim Sci, Linzhi, TN, Peoples R China

4.Anhui Agr Univ, Coll Anim Sci & Technol, Hefei, Anhui, Peoples R China

关键词： Duck hepatitis a virus type 3; miRNA; Transcriptome; miRNA-mRNA network; Innate immune response; Host-virus interactions

期刊名称：BMC GENOMICS （ 影响因子：3.969； 五年影响因子：4.478 ）

ISSN： 1471-2164

年卷期： 2020 年 21 卷 1 期

页码：

收录情况： SCI

摘要： Background Duck hepatitis A virus type 3 (DHAV-3) is one of the most harmful pathogens in the duck industry. However, the molecular mechanism underlying DHAV-3 infection in ducklings remains poorly understood. To study the genetic regulatory network for miRNA-mRNA and the signaling pathways involved in DHAV-3 infection in ducklings, we conducted global miRNA and mRNA expression profiling of duckling liver tissues infected with lethal DHAV-3 by high-throughput sequencing. Results We found 156 differentially expressed miRNAs (DEMs) and 7717 differentially expressed genes (DEGs) in livers of mock-infected and DHAV-3-infected duckling. A total of 19,606 miRNA-mRNA pairs with negatively correlated expression patterns were identified in miRNA-mRNA networks constructed on the basis of these DEMs and DEGs. Moreover, immune-related pathways, including the cytokine-cytokine receptor interaction, apoptosis, Toll-like receptor, Jak-STAT, and RIG-I-like receptor signaling pathway, were significantly enriched through analyzing functions of mRNAs in the network in response to DHAV-3 infection. Furthermore, apl-miR-32-5p, apl-miR-125-5p, apl-miR-128-3p, apl-miR-460-5p, and novel-m0012-3p were identified as potential regulators in the immune-related signaling pathways during DHAV-3 infection. And some host miRNAs were predicted to target the DHAV-3 genome. Conclusions This is the first integrated analysis of miRNA and mRNA in DHAV-3-infected ducklings. The results indicated the important roles of miRNAs in regulating immune response genes and revealed the immune related miRNA-mRNA regulation network in the DHAV-3-infected duckling liver. These findings increase our knowledge of the roles of miRNAs and their target genes in DHAV-3 replication and pathogenesis. They also aid in the understanding of host-virus interactions.

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