Development of double-antibody sandwich ELISA for rapidly quantitative detection of antigen concentration in inactivated SCRV vaccine
文献类型： 外文期刊
作者： Niu, Yinjie 1 ; Zhang, Peng 2 ; Wang, Luyao 1 ; Li, Ningqiu 1 ; Lin, Qiang 1 ; Liu, Lihui 1 ; Liang, Hongru 1 ; Huang, Zh 1 ;
作者机构： 1.Chinese Acad Fishery Sci, Pearl River Fisheries Res Inst, Key Lab Fishery Drug Dev, Minist Agr & Rural Affairs,Key Lab Aquat Anim Imm, Guangzhou 510380, Peoples R China
2.Dalian Ocean Univ, Coll Fisheries & Life Sci, Dalian 116023, Peoples R China
关键词： Siniperca chuatsi rhabdovirus(SCRV); DAS-ELISA; Quantitative detection; SCRV-QY inactivated vaccines
期刊名称：AQUACULTURE （ 影响因子：4.242； 五年影响因子：4.723 ）
ISSN： 0044-8486
年卷期： 2020 年 520 卷
页码：
收录情况： SCI
摘要： In recent years, Siniperca chuatsi rhabdovirus (SCRV) caused serious threats and huge economic losses in Siniperca chuatsi aquaculture industry. Vaccination is the most efficacious and cost-effective strategy to control this viral disease. However, SCRV-QY vaccine quality control is vital for successful prevention. Herein, we generated a pair of high affinity antibodies against SCRV-QY virus and established a double-antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA) for detecting the SCRV-QY antigen amounts. In this assay, monoclonal antibody 4H8 was selected as capture antibody and 4E12 labeled HRP for detector antibody. A standard curve was generated using the SCRV concentration versus OD value with the linear range of concentration of 78.125-5000 ng/ml. The antigen content of 3 batches SCRV-QY inactivated vaccines were quantitatively detected by using the DAS-ELISA. The results showed that antigen contents of SCRV-QY inactivated vaccines were positively correlated with the viral titers. In conclusion, this DAS-ELISA was an accurate, quick and efficacious method for detecting antigen concentration of inactivated SCRV-QY vaccines.
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