Multiplex microsatellite PCR panels and their application in genetic analyses of bighead carp (Hypophthalmichthys nobilis) and silver carp (H. molitrix)
文献类型： 外文期刊
作者： Fang, Min 1 ; Fu, Jianjun 2 ; Zhu, Wenbin 2 ; Wang, Lanmei 2 ; Jiang, Bingjie 1 ; Dong, Zaijie 1 ;
作者机构： 1.Nanjing Agr Univ, Wuxi Fisheries Coll, Wuxi, Jiangsu, Peoples R China
2.Chinese Acad Fishery Sci, Minist Agr & Rural Affairs, Key Lab Freshwater Fisheries & Germplasm Resourc, Freshwater Fisheries Res Ctr, Wuxi, Jiangsu, Peoples R China
关键词： genetic analysis; Hypophthalmichthys molitrix; Hypophthalmichthys nobilis; microsatellite; multiplex PCR
期刊名称：JOURNAL OF APPLIED ICHTHYOLOGY （ 影响因子：0.892； 五年影响因子：0.956 ）
ISSN： 0175-8659
年卷期：
页码：
收录情况： SCI
摘要： In this study, three multiplex PCR panels involving twelve microsatellite loci were developed for cross-species amplification in bighead carp (Hypophthalmichthys nobilis) and silver carp (H. molitrix). The panels were verified using population genetic analyses, and species and simulated hybrid discriminations based on the genotype data of all individuals from two populations for each species. The number of alleles (Na), the observed and expected heterozygosity (Ho and He), and the polymorphic information content (PIC) of twelve loci were ranged from 5 to 20, 0.189 to 0.956, 0.177 to 901, and 0.169 to 0.887, respectively. Overall, almost all the microsatellite loci were detected as highly polymorphic across the two species. The populations of same species showed close genetic distances and similar genetic structures. Significant genetic differentiations were only detected between populations from different species, with a higher variation between species (21.17%) compared with that between populations within species (0.18%). In the species and simulated hybrid discrimination analyses, the individuals from different species were separated distinctly, and the simulated hybrids could almost be separated with the true individuals. In brief, the results indicated the high efficiency of the microsatellite multiplex panels developed in this study, which provide a practicable tool for further genetic analyses of H. nobilis and H. molitrix.
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