文献类型： 外文期刊
作者： Dong, Zaijie 1 ; Luo, Mingkun 2 ; Wang, Lanmei 1 ; Yin, Haoran 2 ; Zhu, Wenbin 1 ; Fu, Jianjun 1 ;
作者机构： 1.Chinese Acad Fishery Sci, Key Lab Freshwater Fisheries & Germplasm Resource, Freshwater Fisheries Res Ctr, Minist Agr & Rural Affairs, Wuxi, Jiangsu, Peoples R China
2.Nanjing Agr Univ, Wuxi Fisheries Coll, Nanjing, Jiangsu, Peoples R China
关键词： MiRNA-206; Mc1r; skin color; melanocytes; koi carp
期刊名称：FRONTIERS IN GENETICS （ 影响因子：4.599； 五年影响因子：4.888 ）
ISSN：
年卷期： 2020 年 11 卷
页码：
收录情况： SCI
摘要： MicroRNAs (miRNAs) are similar to 22 nucleotide non-coding RNA molecules that act as crucial roles in plenty of biological processes. However, the molecular and cellular mechanisms of miRNAs to regulate skin color differentiation and pigmentation in fish have not been fully understood. Herein, we revealed that miR-206, a skin-enriched miRNA, regulates melanocortin 1 receptor (Mc1r, a key regulator of melanogenesis) expression by binding to its 3'-untranslated (UTR) region through bioinformatics and luciferase reporter assay in koi carp (Cyprinus carpio L.). The analysis of spatial and temporal expression patterns suggested that miR-206 is a potential regulator in the skin pigmentation process. Then, we silenced it in vivo with an antagomir method. The result showed a substantial increase of Mc1r mRNA expression and protein level, and also its downstream genes: tyrosinase (Tyr) and dopachrome tautomerase (Dct) that encoding key enzymes involved in melanin synthesis. Moreover, we constructed the miRNA-206 sponge lentivirus vector to transfect koi carp melanocytes in vitro, further checked the functions of melanocytes using Cck-8 and Transwell assays. As a result, inhibition of miR-206 significantly up-regulated Mc1r mRNA expression and protein level and accelerated the melanocyte proliferation and migration ability compared with the scrambled-sequence negative control group (miR-NC). Overall, these findings provide the evidence that miR-206 plays a regulatory role in the skin color pigmentation through targeting the Mc1r gene and would facilitate understanding the molecular regulatory mechanisms underlying miRNA-mediated skin color pigmentation in koi carp.
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