您好，欢迎访问中国水产科学研究院 机构知识库！
中国水产科学研究院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Oral delivery of Bacillus subtilis spores expressing Clonorchis sinensis paramyosin protects grass carp from cercaria infection

文献类型： 外文期刊

作者： Sun, Hengchang 1 ; Shang, Mei 1 ; Tang, Zeli 5 ; Jiang, Hongye 2 ; Dong, Huimin 1 ; Zhou, Xinyi 2 ; Lin, Zhipeng 2 ; Shi, 1 ;

作者机构： 1.Sun Yat Sen Univ, Affiliated Hosp 3, Dept Lab Med, Guangzhou, Peoples R China

2.Sun Yat Sen Univ, Zhongshan Sch Med, Dept Parasitol, Guangzhou, Peoples R China

3.Sun Yat Sen Univ, Key Lab Trop Dis Control, Minist Educ, Guangzhou, Guangdong, Peoples R China

4.Prov Engn Technol Res Ctr Biol Vector Control, Guangzhou 510080, Guangdong, Peoples R China

5.Guangxi Med Univ, Sch Preclin Med, Dept Cell Biol & Genet, Nanning 530021, Peoples R China

6.Chinese Acad Fishery Sci, Fisheries Res Inst, Key Lab Fishery Drug Dev, Pearl River,Minist Agr, Guangzhou, Guangdong, Peoples R China

关键词： Clonorchis sinensis; Bacillus subtilis spore; Paramyosin; Oral vaccine; Intestinal microbiota; Grass carp

期刊名称：APPLIED MICROBIOLOGY AND BIOTECHNOLOGY （ 影响因子：4.813； 五年影响因子：4.697 ）

ISSN： 0175-7598

年卷期： 2020 年 104 卷 4 期

页码：

收录情况： SCI

摘要： Clonorchis sinensis (C. sinensis), an important fishborne zoonotic parasite threatening public health, is of major socioeconomic importance in epidemic areas. Effective strategies are still urgently expected to prevent against C. sinensis infection. In the present study, paramyosin of C. sinensis (CsPmy) was stably and abundantly expressed on the surface of Bacillus subtilis spores. The recombinant spores (B.s-CotC-CsPmy) were incorporated in the basal pellets diet in three different dosages (1 x 10(5), 1 x 10(8), 1 x 10(11) CFU/g pellets) and orally administrated to grass carp (Ctenopharyngodon idella). The immune responses and intestinal microbiota in the treated grass carp were investigated. Results showed that specific anti-CsPmy IgM levels in sera, skin mucus, bile, and intestinal mucus, as well as mRNA levels of IgM and IgZ in the spleen and head kidney, were significantly increased in B.s-CotC-CsPmy-10(11) group. Besides, transcripts levels of IL-8 and TNF-alpha in the spleen and head kidney were also significantly elevated than the control groups. Moreover, mRNA levels of tight junction proteins in the intestines of B.s-CotC-CsPmy-10(11) group increased. Potential pathogenetic bacteria with lower abundance and higher abundances of candidate probiotics and bacteria associated with digestion in 1 x 10(11) CFU/g B.s-CotC-CsPmy spores administrated fishes could be detected compared with control group. The amount of metacercaria in per gram fish flesh was statistically decreased in 1 x 10(11) CFU/g B.s-CotC-CsPmy spores orally immunized group. Our work demonstrated that B. subtilis spores presenting CsPmy on the surface could be a promising effective, safe, and needle-free candidate vaccine against C. sinensis infection for grass carp.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：中国水产科学研究院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台