您好，欢迎访问浙江省农业科学院 机构知识库！
浙江省农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

A highly integrated system with rapid DNA extraction, recombinase polymerase amplification, and lateral flow biosensor for on-site detection of genetically modified crops

文献类型： 外文期刊

作者： Wang, Xiaofu 1 ; Chen, Yu 1 ; Chen, Xiaoyun 1 ; Peng, Cheng 1 ; Wang, Liu 1 ; Xu, Xiaoli 1 ; Wu, Jian 3 ; Wei, Wei 1 ; Xu, J 1 ;

作者机构： 1.Zhejiang Acad Agr Sci, State Key Lab Qual & Safety Agroprod, Hangzhou 310021, Peoples R China

2.Minist Agr China, Key Lab Informat Traceabil Agr Prod, Hangzhou 310021, Peoples R China

3.Zhejiang Univ, Coll Biosyst Engn & Food Sci, Hangzhou 310058, Peoples R China

4.Shenyang Normal Univ, Coll Chem & Life Sci, Shenyang 110034, Peoples R China

关键词： On-site detection; Recombinase polymerase amplification; Lateral flow biosensor; Genetically modified crops

期刊名称：ANALYTICA CHIMICA ACTA （ 影响因子：6.558； 五年影响因子：6.228 ）

ISSN： 0003-2670

年卷期： 2020 年 1109 卷

页码：

收录情况： SCI

摘要： With the large-scale planting of genetically modified (GM) crops, the development of a rapid and convenient method for on-site monitoring GM crops is needed. In this study, a duplex recombinase polymerase amplification (DRPA)-based, quick and simple detection system is presented for on-site detection of GM crops. In this system, a rapid DNA extraction method, a DRPA, and a lateral flow biosensor (LFB) are integrated to allow for rapid DNA extraction and amplification, and fast visualization of the detection results. Using the rapid DNA extraction method, high-quality DNA suitable for RPA and polymerase chain reaction (PCR) was quickly isolated from various crops within 5 min. Utilizing the optimal DRPA assay, the universal screening sequences (Cauliflower mosaic virus 35S promoter [35S] and Agrobacterium tumefaciens NOS terminator [NOS]) were rapidly and simultaneously amplified with high selectivity and sensitivity. The sensitivity threshold of the DRPA assay was -10 copies for GM soybean genomic DNA and 100 ng DNA of 0.1% GM soybean. In combination with the LFB in an enclosed cassette, the entire detection process was performed in approximately 20-30 min and eliminated the carryover contamination. No special or expensive equipment was needed for the detection process. The system was successfully applied and validated for on-site detection of GM rice, demonstrating its suitability for onsite testing of GM crops and high potential for application to other fields, especially in low-resource regions that require rapid detection of DNA targets. (C) 2020 Elsevier B.V. All rights reserved.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：浙江省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台