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Broad-spectrum detection of zeranol and its analogues by a colloidal gold-based lateral flow immunochromatographic assay in milk

文献类型： 外文期刊

作者： Yin, Mengqi 1 ; Hu, Xiaofei 2 ; Sun, Yaning 2 ; Xing, Yunrui 2 ; Xing, Guangxu 2 ; Wang, Yanwei 2 ; Li, Qingmei 2 ; Wang, 1 ;

作者机构： 1.Jiangnan Univ, Sch Food Sci & Technol, Wuxi 214122, Jiangsu, Peoples R China

2.Henan Acad Agr Sci, Key Lab Anim Immunol, Zhengzhou 450002, Peoples R China

3.Henan Univ Sci & Technol, Coll Food & Bioengn, Luoyang 471023, Peoples R China

4.Henan Agr Univ, Coll Anim Sci & Vet Med, Zhengzhou 450002, Peoples R China

关键词： Zeranol; Immunochromatographic strip; Colloidal gold; Broad-spectrum; Milk

期刊名称：FOOD CHEMISTRY （ 影响因子：7.514； 五年影响因子：7.516 ）

ISSN： 0308-8146

年卷期： 2020 年 321 卷

页码：

收录情况： SCI

摘要： Based on colloidal gold and broad-spectrum monoclonal antibody that binds to zeranol and its five analogues with high sensitivity, a lateral flow immunochromatographic assay (LFIA) in a competitive format was developed to specifically determine residues of zeranol, an illegal growth promoter in livestock. In this study, the assay had high sensitivity and was broad-spectrum only for zeranol and its five analogues, and the results were obtained within 10 min without needing sophisticated procedures. The cutoff values for zeranol and its five analogues were 10 ng/mL, and the IC50 values for zeranol, beta-zearalanol, zearalanone, alpha-zearalenol, beta-zearalenol and zearalenone were 1.250, 1.800, 1.775, 1.225, 1.709 and 1.319 ng/mL, respectively. The recovery rates were ranged from 85.6 to 93.9%, with the coefficient of variations less than 12.4%. The results demonstrated that the LFIA could be used for rapid, simultaneous, semi-quantitative and quantitative detection of residues of zeranol and its five analogous in milk.

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