您好，欢迎访问吉林省农业科学院 机构知识库！
吉林省农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

A fractionation procedure for identifying novel proteins induced by chill stress in Arabidopsis thaliana

文献类型： 外文期刊

作者： Sun, Liwen 1 ; Wang, Shichen 1 ; Xi, Jinghui 2 ; Yang, Shuo 1 ; Liu, Xiangguo 1 ; Chai, Xin 1 ; Xin, Hongbao 1 ; An, Baiy 1 ;

作者机构： 1.Jilin Univ, Minist Educ, Key Lab Mol Enzymol & Engn, Changchun 130023, Peoples R China

2.Jilin Univ, Coll Plant Sci, Changchun 130062, Peoples R China

关键词： Arabidopsis;Chill stress;Debris;Fractionation;Salt gradient fraction

期刊名称：BIOTECHNOLOGY LETTERS （ 影响因子：2.461； 五年影响因子：2.457 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Extraction of plant proteins using typical extraction buffers leaves insoluble debris that cannot be investigated by conventional 2-DE technologies. In this paper, we present a scalable, off-line procedure for extraction of Arabidopsis thaliana homogenates that can be used in combination with both in-gel digestion and mass spectrometry. Based on sequential NaCl gradients and strong detergent fractionation, this new strategy allowed detection of 11 novel proteins from Arabidopsis thaliana that were altered in response to chilling stress.

  • 相关文献

[1]Expression and activity analysis of beta Gallinacin-3 in Arabidopsis. Jin, Libo,Wang, Yunpeng,Xu, Nuo,Wang, Dezhong,Peng, Renyi,Jiang, Chao,Li, Xiaokun,Jin, Libo,Liu, Xiuming,Jiang, Chao,Wang, Yunpeng,Li, Xiaokun. 2018

[2]Molecular dynamics simulations reveal the disparity in specific recognition of GCC-box by AtERFs transcription factors super family in Arabidopsis. Wang, Shichen,Yang, Shuo,Hao, Dongyun,Yin, Yuejia,Hao, Dongyun,Xi, Jinghui,Li, Shanyu. 2009

[3]ZmDBF3, a Novel Transcription Factor from Maize (Zea mays L.), Is Involved in Multiple Abiotic Stress Tolerance. Zhou, Wei,Jia, Cheng-Guo,Wu, Xian,Yu, Gang,Zhang, Xiang-Hui,Liu, Jin-Liang,Pan, Hong-Yu,Zhou, Wei,Hu, Rui-Xue,Wu, Xian.

作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：吉林省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台