Prokaryotic taxonomy and functional diversity assessment of different sequencing platform in a hyper-arid Gobi soil in Xinjiang Turpan Basin, China
文献类型： 外文期刊
作者： Zhang, Zhidong 1 ; Zhu, Jing 1 ; Ghenijan, Osman 1 ; Chen, Jianwei 2 ; Wang, Yuxian 3 ; Jiang, Ling 3 ;
作者机构： 1.Xinjiang Acad Agr Sci, Inst Appl Microbiol, Xinjiang Key Lab Special Environm Microbiol, Urumqi, Peoples R China
2.BGI Res, Qingdao, Peoples R China
3.Nanjing Tech Univ, Coll Food Sci & Light Ind, Nanjing, Peoples R China
4.Nanjing Tech Univ, State Key Lab Mat Oriented Chem Engn, Nanjing, Peoples R China
关键词： Turpan Basin; next generation sequencing; hyper-arid soil; prokaryotic diversity; community assembly
期刊名称：FRONTIERS IN MICROBIOLOGY （ 影响因子：5.2； 五年影响因子：6.2 ）
ISSN：
年卷期： 2023 年 14 卷
页码：
收录情况： SCI
摘要： Turpan Basin located in the eastern Xinjiang is a typical arid inland basin with extremely scarce water resources and a fragile ecosystem. Prokaryotic communities with unique genetic and physiological modifications can survive and function in such harsh environments, offering diverse microbial resources. However, numerous microbes can enter the viable but non-culturable state because of drought stress in the desert soil. In this work, next generation sequencing (NGS) technology based on DNA nanoball sequencing platform (DNBSEQ-G400) and sequencing-by-synthesis platform (NovaSeq 6000) were applied to analyze the prokaryotic diversity in three hyper-arid Gobi soils from Flaming Mountain, Toksun, and Kumtag. The comparison between two platforms indicated that DNBSEQ-G400 had better repeatability and could better reflect the prokaryotic community of this hyper-arid region. The diversity analysis based on DNBSEQ-G400 identified a total of 36 bacterial phyla, including Pseudomonadota, Bacteroidota, Bacillota, Actinomycetota, Methanobacteriota, Acidobacteriota, Nitrososphaerota, and Planctomycetota. The environmental factors, including soluble salt, available potassium, total nitrogen, and organic matter, were positively correlated with the abundance of most prokaryote. In addition, the prokaryotic community assembly in hyper-arid soil was well described by neutral-based models, indicating that the community assembly was mainly controlled by stochastic processes. Finally, the phylogenetic analysis of Actinomycetota proved that such extremophiles played an important role in the ecosystems they colonize. Overall, our result provides a reference for choosing the appropriate sequencing platform and a perspective for the utilization of soil microbial resources from hyper-arid regions.
- 相关文献
作者其他论文 更多>>
-
Preparation and evaluation of novel Agriophyllum squarrosum starch nanoparticles for encapsulation of lycopene with enhanced retention rate and bioactivity during simulated in-vitro digestion
作者：Cao, Shaopan;Li, Senqiao;Zhang, Liling;Jiang, Ling;Jiang, Ling;Zhang, Zhidong
关键词：Starch nanoparticles; Ionic liquids; Lycopene; Encapsulation; Bioactivity retention
-
Application of Synthetic Microbial Communities of Kalidium schrenkianum in Enhancing Wheat Salt Stress Tolerance
作者：Zhu, Jing;Jia, Qiong;Tang, Qi-Yong;Osman, Ghenijan;Gu, Mei-Ying;Wang, Ning;Zhang, Zhi-Dong
关键词：
Kalidium schrenkianum ; synthetic microbial communities; wheat; salt stress tolerance -
Microbe-mediated synthesis of defect-rich CeO2 nanoparticles with oxidase-like activity for colorimetric detection of
l -penicillamine and glutathione作者：Zhang, Nan;Du, Yingyan;Zhu, Liying;Jiang, Ling;Jiang, Ling;Zhang, Zhidong
关键词：
-
Screening, identification, metabolic pathway of di-n-butyl phthalate degrading Priestia megaterium P-7 isolated from long-term film mulched cotton field soil in Xinjiang
作者：Yi, Yuanyang;Zhang, Wei;Zhang, Zhidong;Wang, Yuxian;Liu, Wanqin;Zhang, Zhidong;Zhu, Jing;Gu, Meiying;Jia, Qiong;Zhang, Zhidong;Li, Xue;Mutalifu, Munire;Zhang, Zhidong;Jiang, Ling
关键词：Di-n-butyl phthalate; biodegradation; whole-genome sequencing; metabolomics analysis;
Priestia megaterium P-7 -
Chryseobacterium gossypii sp. nov., a bacterium isolated from long-term continuous cropping cotton fields
作者：Mulati, Mila;Kong, Delong;Chai, Lili;Zhang, Zhidong;Zhang, Wei;Yi, Yuanyang
关键词：16S rRNA gene; Chryseobacterium; genome; polyphasic taxonomy
-
Isolation and characterization of a novel Paenibacillus species from high-salt environment with broad-spectrum antimicrobial activity against food spoilage microorganisms
作者：Han, Fei;Tong, Danni;Guo, Zixuan;Li, Zhen;Du, Rubing;Xu, Qing;Zhang, Zhidong
关键词：Food safety; Paenibacillus sp. C42; Secondary metabolites; Extreme environments; Antimicrobial activity
-
Transforming Poly(butylene adipate-co-terephthalate) into Protocatechuic Acid via Enzymatic Degradation and Whole-Cell Catalysis
作者：Li, Yunhan;Su, Can;Hu, Fanghui;Ling, Jiahuan;Zhang, Zhidong;Zhang, Lihui;Zhang, Zhidong
关键词：
