文献类型： 外文期刊

作者： Yuan, Min ¹ ; Cong, Fangdi ¹ ; Zhai, Yali ¹ ; Li, Ping ¹ ; Yang, Wei ¹ ; Zhang, Shulin ¹ ; Su, Yongpeng ² ; Li, Tao ³ ; Wang ¹ ;

作者机构： 1.Tianjin Agr Univ, Coll Basic Sci, Tianjin Chem Expt Teaching Demonstrat Ctr, Tianjin Key Lab Aqua Ecol & Aquaculture, Tianjin 300392, Peoples R China

2.Biccamin Tianjin Biotechnol R&D Stock Co Ltd, Tianjin 300393, Peoples R China

3.Xinxiang Med Univ, Sch Life Sci & Technol, Xinxiang 453003, Henan, Peoples R China

4.Tianjin Acad Agr Sci, Tianjin Key Lab Crop Genet & Breeding, Crop Inst, Tianjin 300384, Peoples R China

关键词： Citronellyl acetate; Pseudomonas fluorescens lipase; Immobilization; Nonaqueous catalysis; Rice straw

期刊名称：BIOPROCESS AND BIOSYSTEMS ENGINEERING （ 影响因子：3.21； 五年影响因子：2.97 ）

ISSN： 1615-7591

年卷期：

页码：

收录情况： SCI

摘要： Citronellyl acetate as an important flavor, can be effectively synthesized by lipase catalysis in nonaqueous system. But lipases usually behave low catalytic activity due to aggregation and denaturation of them in organic phase. To enhance the nonaqueous catalysis, based on the mechanism of lipases activated at water/oil (organic phase) interface, the inexpensive race straw was processed into powder and filaments on which Pseudomonas fluorescens lipase was immobilized by physical adsorption, used for synthesis of citronellyl acetate via transesterification of citronellol and vinyl acetate. Results showed that the desired loading was 10 mg lipase immobilized on 30 mg rice straw filaments or 25 mg rice straw powder. When the two immobilized lipases were employed in the reaction system consisted of 1-mL citronellol and 2-mL vinyl acetate at 37 celcius and 160 rpm, the conversions all reached 99.8% after 12 h. Under the reaction condition, the conversion catalyzed by 10 mg native lipase was 85.1%. Undergoing six times of 8-h reuses in the organic system, the filament and power immobilized lipases had weak activity attenuation rates 0.36 and 0.32% h(-1), lower than 1.52% h(-1) of native lipase. Even at the room temperature and the static state without shaking and stirring, the rice straw filaments immobilized lipase could brought conversion 62.9% after 10 h but the native lipase only gave 37.0%. Obviously, the rice straw, especially its filaments, is an inexpensive and available natural material to prepare immobilized lipase with desired catalysis in organic phase, meant significant potential in flavor industry.