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The differential expression of peroxisome proliferators-activated receptors in various duck tissues

文献类型： 外文期刊

作者： Wu, Yan 1 ; Liu, Xiaolin 1 ; Xiao, Hongwei 2 ; Zhang, Huilin 1 ;

作者机构： 1.NW A&F Univ, Coll Anim Sci & Technol, Shaanxi Key Lab Mol Biol Agr, Yangling 712100, Shaanxi, Peoples R China

2.Hubei Acad Agr Sci, Inst Anim Husb & Vet, Wuhan 430064, Peoples R China

关键词： Peking duck (Anas platyrhynchos);PPARα;PPARγ;Clone;Expression pattern

期刊名称：MOLECULAR BIOLOGY REPORTS （ 影响因子：2.316； 五年影响因子：2.357 ）

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收录情况： SCI

摘要： Peroxisome proliferators-activated receptors (PPARs) plays important role in regulate a variety of target genes which involves in lipid metabolism. In the present study, 1,430 bp of PPARl and 1,650 bp of PPARd in Peking duck (Anas platyrhynchos) were cloned by reverse transcription (RT)-PCR. The cloned sequences of PPARl and PPARd were highly conserved, although relevant changes with respect to human, mouse, chicken, and goose were identified. Real-time quantitative RT-PCR was used to determine if the PPARl and PPARd were expressed in different tissues of male and female Peking ducks. The expression of PPARl and PPARd were widely distributed in all examined tissues. The expression level of PPARl was highest in liver and lowest in kidney in male and female ducks. And it was highest in abdominal fat and lowest in small intestine of PPARd in male and female ducks.

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