文献类型： 外文期刊
作者： Guo, Rihong 1 ; Wang, Huili 1 ; Meng, Chunhua 1 ; Gui, Hongbing 1 ; Li, Yinxia 1 ; Chen, Fang 1 ; Zhang, Chenjian 1 ; Zhang, Han 1 ; Ding, Qiang 1 ; Zhang, Jianli 1 ; Zhang, Jun 1 ; Qian, Yong 1 ; Zhong, Jifeng 1 ; Cao, Shaoxian 1 ;
作者机构： 1.Jiangsu Prov Engn Res Ctr Precis Anim Breeding, Nanjing 210014, Peoples R China
2.Jiangsu Acad Agr Sci, Inst Anim Sci, Nanjing 210014, Peoples R China
3.Minist Agr & Rural Affairs, Key Lab Crop & Anim Integrated Farming, Nanjing 210014, Peoples R China
关键词： CRISPR; Cas9; Hu sheep; MSTN; double muscling phenotype; off-target effects
期刊名称：GENES （ 影响因子：3.5； 五年影响因子：3.9 ）
ISSN：
年卷期： 2023 年 14 卷 6 期
页码：
收录情况： SCI
摘要： Hu sheep, an indigenous breed in China known for its high fecundity, are being studied to improve their growth and carcass traits. MSTN is a negative regulator of muscle development, and its inactivation results in muscularity. The C-CRISPR system, utilizing multiple neighboring sgRNAs targeting a key exon, has been successfully used to generate genes for complete knockout (KO) monkeys and mice in one step. In this study, the C-CRISPR system was used to generate MSTN-edited Hu sheep; 70 embryos injected with Cas9 mRNA and four sgRNAs targeting exon 3 of sheep MSTN were transferred to 13 recipients. Out of 10 lambs born from five recipients after full-term pregnancies, nine had complete MSTN KO with various mutations. No off-target effects were found. These MSTN-KO Hu sheep showed a double-muscled (DM) phenotype, characterized by a higher body weight at 3 and 4 months old, prominent muscular protrusion, clearly visible intermuscular groves, and muscle hypertrophy. The molecular analysis indicated enhanced AKT and suppressed ERK1/2 signaling in the gluteus muscle of the edited Hu sheep. In conclusion, MSTN complete KO Hu sheep with a DM phenotype were efficiently and specifically generated using C-CRISPR, and the C-CRISPR method is a promising tool for farm animal breeding.
- 相关文献
作者其他论文 更多>>
-
miR-21/SMAD2 Is Involved in the Decrease in Progesterone Synthesis Caused by Lipopolysaccharide Exposure in Follicular Granulosa Cells of Laying Goose
作者：Guo, Xinyi;Ying, Shijia;Xiao, Huiping;An, Hao;Wu, Wenda;Guo, Xinyi;Xiao, Huiping;Wu, Wenda;Guo, Xinyi;Ying, Shijia;An, Hao;Guo, Rihong;Dai, Zichun
关键词：lipopolysaccharide; granulosa cells; miR-21; progesterone; goose
-
Integrated Effects of Straw Incorporation and N Application on Rice Yield and Greenhouse Gas Emissions in Three Rice-Based Cropping Systems
作者：Bankole, Oluwaseyi Oyewale;Danso, Frederick;Zhang, Nan;Zhang, Jun;Zhang, Xin;Li, Gexing;Raheem, Abdulkareem;Deng, Aixing;Zheng, Chengyan;Song, Zhenwei;Zhang, Weijian;Zhang, Nan;Zhang, Kun;Dong, Wenjun;Lu, Changying;Li, Gexing;Raheem, Abdulkareem
关键词：straw; N fertilizer; rice yield; CH4 emissions; yield-scaled emissions; mcrA; cropping systems
-
Improving duckling hatchability and quality by optimization of egg turning angle during incubation
作者：Chen, Fang;Zhu, Biwen;Guo, Binbin;Dai, Zichun;Ying, Shijia;Shi, Zhendan;Chen, Fang;Zhu, Biwen;Guo, Binbin;Dai, Zichun;Ying, Shijia;Shi, Zhendan;Zhu, Biwen;Huang, Yunmao;Shi, Zhendan
关键词：duck; egg turning angle; incubation; hatchability; growth performance
-
Effects of ambient temperature on growth performance, slaughter traits, meat quality and serum antioxidant function in Pekin duck
作者：Xu, Congcong;Sun, Dongyue;Liu, Yi;Pan, Ziyi;Dai, Zichun;Chen, Fang;Guo, Rihong;Chen, Rong;Shi, Zhendan;Ying, Shijia;Xu, Congcong;Sun, Dongyue;Liu, Yi;Shi, Zhendan;Ying, Shijia
关键词：Pekin duck; temperature; growth performance; slaughtering traits; meat quality; antioxidant function
-
Genetically Engineered Goats as Efficient Mammary Gland Bioreactors for Production of Recombinant Human Neutrophil Peptide 1 Using CRISPR/Cas9
作者：Li, Dongxu;Wang, Jingang;Wang, Feng;Wan, Yongjie;Guo, Rihong;Chen, Fang;Guo, Rihong;Chen, Fang
关键词：CRISPR/Cas9; goat; HNP1; beta-casein; antibacterial activity
-
The role of miR-10a-5p in LPS-induced inhibition of progesterone synthesis in goose granulosa cells by down-regulating CYP11A1
作者：Guo, Xinyi;An, Hao;Ying, Shijia;Wu, Wenda;Guo, Xinyi;An, Hao;Guo, Rihong;Dai, Zichun;Ying, Shijia;Guo, Xinyi;Wu, Wenda
关键词：LPS; goose; miR-10a-5p; granulosa cells; progesterone
-
The Impact of Different Relative Humidity Levels on the Production Performance, Slaughter Performance, and Meat Quality of White Pekin Ducks Aged 4 to 42 Days
作者：Sun, Dongyue;Ying, Shijia;Sun, Dongyue;Xu, Congcong;Liu, Yi;Dai, Zichun;Pan, Ziyi;Chen, Rong;Guo, Rihong;Chen, Fang;Shi, Zhendan;Ying, Shijia;Xu, Congcong;Liu, Yi
关键词：Pekin ducks; production performance; meat quality; humidity
