Transcriptomic and proteomic analysis reveals (E)-2-hexenal modulates tomato resistance against Botrytis cinerea by regulating plant defense mechanism
文献类型： 外文期刊
作者： Zhang, Jihong 1 ; Li, Yuqiong 1 ; Du, Shenglong 2 ; Deng, Zhiping 3 ; Liang, Quanwu 1 ; Song, Ge 1 ; Wang, Haihua 1 ; Yan, Mingli 1 ; Wang, Xuewen 4 ;
作者机构： 1.Hunan Univ Sci & Technol, Coll Life Sci, Hunan Key Lab Econ Crops Genet Improvement & Integ, Xiangtan 411201, Peoples R China
2.Xiangtan Univ, Coll Chem Engn & Technol, Xiangtan 411105, Peoples R China
3.Zhejiang Acad Agr Sci, Inst Virol & Biotechnol, Hangzhou 310022, Peoples R China
4.Univ Georgia, Dept Genet, Athens, GA 30602 USA
关键词： (E)-2-hexenal; Botrytis cinerea; Lesion diameters; Phenylpropanoid biosynthesis; Pathogenesis-related proteins; Solanum lycopersicum
期刊名称：PLANT MOLECULAR BIOLOGY （ 影响因子：5.1； 五年影响因子：5.3 ）
ISSN： 0167-4412
年卷期： 2023 年 111 卷 6 期
页码：
收录情况： SCI
摘要： In a previous study, we observed that (E)-2-hexenal stimulated systemic resistance against B. cinerea in tomato plants. However, the molecular mechanisms underlying (E)-2-hexenal-mediated regulation of systemic immunity against B. cinerea remained unclear. In the current study, the global mechanism underlying (E)-2-hexenal-meidated regulation of biotic stress tolerance in tomato was investigated using RNA-seq- and LC-MS/MS- integrated transcriptomic and proteomic analyses. Compared to control plants, (E)-2-hexenal-treated plants exhibited reduced susceptibility to B. cinerea, with a 50.51% decrease in lesion diameters. Meanwhile, (E)-2-hexenal vapor fumigation significantly increased total phenolic content and activities of various antioxidant enzymes peroxidase (POD), phenylalanine ammonia lyase (PAL), and lipoxygenase (LOX). A total of 233 differentially expressed genes (DEGs) and 400 differentially expressed proteins (DEPs), respectively, were identified. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis revealed that (E)-2-hexenal treatment markedly affected the expression of genes involved in multiple metabolic pathways, especially glutathione metabolism, phenylpropanoid biosynthesis, plant hormone signal transduction, and MAPK signaling pathway. Notably, proteomic analysis revealed modulation of the activities of several defense response proteins, such as pathogenesis-related (PR) proteins (Solyc02g031950.3.1, Solyc02g031920.4.1, and Solyc04g064870.3.1), peroxidases (Solyc06g050440.3.1, Solyc01g105070.3.1, Solyc01g015080.3.1, Solyc03g025380.3.1 and Solyc06g076630.3.1). Our results provide a comprehensive analysis of the effects of (E)-2-hexenal treatment on the transcriptome and proteome of tomato plants, which might be used as a reference in further studies on plant defense responses against pathogens.
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