文献类型： 外文期刊

作者： Shi, Ji-Feng ¹ ; Cheng, Man-hong ⁶ ; Zhou, Wei ³ ; Zeng, Mu-zi ⁵ ; Chen, Yu ⁵ ; Yang, Jia-xin ⁵ ; Wu, Hao ⁵ ; Ye, Qiu-hong ⁵ ; Tang, Hong ⁵ ; Zhang, Qing ³ ; Fu, Kai-Yun ⁷ ; Guo, Wen-Chao ² ;

作者机构： 1.Southwest Univ, Coll Sericulture Text & Biomass Sci, State Key Lab Resource Insects, Chongqing, Peoples R China

2.Xinjiang Acad Agr Sci, Inst Plant Protect, Urumqi, Peoples R China

3.Southwest Univ, State Key Lab Resource Insects, Chongqing, Peoples R China

4.Minist Agr & Rural Affairs, Key Lab Sericultural Biol & Genet Breeding, Chongqing, Peoples R China

5.Southwest Univ, Coll Sericulture Text & Biomass Sci, Chongqing, Peoples R China

6.Chongqing Coll Humanities Sci & Technol, Chongqing, Peoples R China

7.Xinjiang Acad Agr Sci, Inst Plant Protect, Key Lab Integrated Pest Management Crops Northwest, Xinjiang Key Lab Agr Biosafety,Minist Agr, Urumqi, Peoples R China

关键词： chitinase; RNA interference; elytra; veins; Leptinotarsa decemlineata

期刊名称：PEST MANAGEMENT SCIENCE （ 影响因子：4.1； 五年影响因子：4.4 ）

ISSN： 1526-498X

年卷期： 2024 年

页码：

收录情况： SCI

摘要： BACKGROUND The Colorado potato beetle (CPB), Leptinotarsa decemlineata, is a major potato (Solanum tuberosum) pest, infesting over 16 million km2 and causing substantial economic losses. The insect cuticle forms an apical extracellular matrix (ECM) envelope covering exposed organs to direct morphogenesis and confer structural protection. While select chitinase (Cht) genes have proven essential for larval development, their potential activities directing ECM remodeling underlying adult wing maturation remain undefined. RESULTS We investigated the expression patterns and performed an oral RNA interference (RNAi) screen targeting 19 LdChts in late-instar L. decemlineata larvae. Subsequently, we assessed their effects on adult eclosion and wing characteristics. Knockdown of LdCht5, LdCht7, LdCht10, LdIDGF2, and LdIDGF4, as well as others from Group IV (LdCht15, LdCht12, LdCht17, and LdCht13) and Groups VII-X (LdCht2, LdCht11, LdCht1, and LdCht3), resulting in shrunken, misshapen elytra with reduced areal density, as well as transverse wrinkling and impaired wing-tip folding in hindwings. Scanning electron micrographs revealed eroded elytral ridges alongside thinned, ruptured hindwing veins, indicative of mechanical fragility post-LdCht suppression. Spectroscopic analysis uncovered biomolecular alterations underlying the elytral anomalies, including decreases in peaks representing chitin, proteins, and lipids. This loss of essential ECM components provides evidence for the fragility, wrinkling, and shrinkage observed in the RNAi groups. CONCLUSION Our findings elucidate the crucial role of chitinases in the turnover of chitinous cuticles on beetle wings, offering insights into RNAi-based control strategies against this invasive pest. (c) 2024 Society of Chemical Industry.