您好，欢迎访问新疆农业科学院 机构知识库！
新疆农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

RNA interference targeting Ras GTPase gene Ran causes larval and adult lethality in Leptinotarsa decemlineata

文献类型： 外文期刊

作者： Shen, Chen-Hui 1 ; Jin, Lin 1 ; Fu, Kai-Yun 2 ; Guo, Wen-Chao 4 ; Li, Guo-Qing 1 ;

作者机构： 1.Nanjing Agr Univ, Coll Plant Protect, Agr Minist,Key Lab Integrated Pest Management Cro, Dept Entomol,State & Local Joint Engn Res Ctr Gre, Nanjing 210095, Peoples R China

2.Xinjiang Acad Agr Sci, Inst Plant Protect, Urumqi, Peoples R China

3.Minist Agr, Key Lab Intergraded Management Harmful Crop Vermi, Urumqi, Peoples R China

4.Xinjiang Acad Agr Sci, Inst Microbiol Applicat, Urumqi, Peoples R China

关键词： RNA interference; dietary introduction; bacteria-expressed dsRNA; LdRan

期刊名称：PEST MANAGEMENT SCIENCE （ 影响因子：4.462； 五年影响因子：4.688 ）

ISSN： 1526-498X

年卷期： 2022 年 78 卷 9 期

页码：

收录情况： SCI

摘要： BACKGROUND RNA interference (RNAi) is a breakthrough technology in pest control. It is highly efficient to Coleopteran pests such as the Colorado potato beetle Leptinotarsa decemlineata, a serious pest defoliator mainly attacking potatoes worldwide. The first step for effective pest control by RNAi is the development of effective and reliable target genes. RESULTS Our results revealed that continuous ingestion of dsLdRan for 3 days successfully silenced the target gene, inhibited larval growth and killed 100% L. decemlineata larvae. When the bioassay began at the second-, third/fourth-instar larval stages, the larval lethality mainly occurred at the fourth larval instar and prepupal stages, respectively. Importantly, consumption of dsLdRan for 3 days by the newly-emerged males and females effectively knocked down the target transcript, reduced fresh weights and caused 100% of lethality within a week. The LdRan females possessed underdeveloped ovaries. CONCLUSION Considering that the larvae, adults and eggs are simultaneously sited on the potato plants, bacterially-expressed dsLdRan is a potential RNAi-based strategy for managing L. decemlineata in the potato field.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：新疆农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台