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RNA Interference-Mediated Suppression of Ecdysone Signaling Inhibits Choriogenesis in Two Coleoptera Species

文献类型： 外文期刊

作者： Zhang, Xiao-Qing 1 ; Jin, Lin 1 ; Guo, Wen-Chao 2 ; Fu, Kai-Yun 2 ; Li, Guo-Qing 1 ;

作者机构： 1.Nanjing Agr Univ, State & Local Joint Engn Res Ctr Green Pesticide I, Dept Entomol, Coll Plant Protect,Educ Minist,Key Lab Integrated, Nanjing 210095, Peoples R China

2.Minist Agr, Key Lab Intergraded Pest Management Crops Northwes, Urumqi 830091, Peoples R China

3.Xinjiang Acad Agr Sci, Inst Plant Protect, Xinjiang Key Lab Agr Biosafety, Urumqi 830091, Peoples R China

关键词： Leptinotarsa decemlineata; Henosepilachna vigintioctopunctata; choriogenesis; ecdysone signal; chitin-like substance; eggshell

期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：5.6； 五年影响因子：6.2 ）

ISSN： 1661-6596

年卷期： 2024 年 25 卷 8 期

页码：

收录情况： SCI

摘要： During choriogenesis in insects, chorion (eggshell) is formed by surrounding follicular epithelial cells in ovarioles. However, the regulatory endocrine factor(s) activating choriogenesis and the effect of chemical components on eggshell deserve further exploration. In two representative coleopterans, a coccinellid Henosepilachna vigintioctopunctata and a chrysomelid Leptinotarsa decemlineata, genes encoding the 20-hydroxyecdysone (20E) receptor heterodimer, ecdysone receptor (EcR) and ultraspiracle (USP), and two chitin biosynthesis enzymes UDP-N-acetylglucosamine pyrophosphorylase (UAP) and chitin synthase (ChS1), were highly expressed in ovaries of the young females. RNA interference (RNAi)-aided knockdown of either HvEcR or Hvusp in H. vigintioctopunctata inhibited oviposition, suppressed the expression of HvChS1, and lessened the positive signal of Calcofluor staining on the chorions, which suggests the reduction of a chitin-like substance (CLS) deposited on eggshells. Similarly, RNAi of LdEcR or Ldusp in L. decemlineata constrained oviposition, decreased the expression of LdUAP1 and LdChS1, and reduced CLS contents in the resultant ovaries. Knockdown of LdUAP1 or LdChS1 caused similar defective phenotypes, i.e., reduced oviposition and CLS contents in the L. decemlineata ovaries. These results, for the first time, indicate that 20E signaling activates choriogenesis in two coleopteran species. Moreover, our findings suggest the deposition of a CLS on the chorions.

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