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Study on the changes of LHR, FSHR and AR with the development of testis cells in Hu sheep

文献类型： 外文期刊

作者： Su, Jie 1 ; Song, Yongli 3 ; Yang, Yanyan 4 ; Li, Zhijun 1 ; Zhao, Feifei 2 ; Mao, Fei 2 ; Wang, Daqing 2 ; Cao, Guifang 2 ;

作者机构： 1.Inner Mongolia Med Univ, Dept Med Neurobiol, Hohhot 010030, Peoples R China

2.Inner Mongolia Agr Univ, Inner Mongolia Key Lab Basic Vet Sci, Hohhot 010018, Peoples R China

3.Inner Mongolia Univ, Res Ctr Anim Genet Resources Mongolia Plateau, Hohhot 010021, Peoples R China

4.Inner Mongolia Acad Agr & Anim Husb Sci, Hohhot 010000, Peoples R China

关键词： Hu sheep testis; AR; FSHR; LHR; Single -cell RNA sequencing

期刊名称：ANIMAL REPRODUCTION SCIENCE （ 影响因子：2.2； 五年影响因子：2.3 ）

ISSN： 0378-4320

年卷期： 2023 年 256 卷

页码：

收录情况： SCI

摘要： The process of testis development in mammals is accompanied by the proliferation and maturation of Sertoli, Leydig and germ cells. Spermatogenesis depends on hormone regulation, which must bind to a receptor to exert its biological effects. The changes in Hu sheep testis cell composition and FSHR, LHR and AR expression during different developmental stages are unclear (newborn, puberty and adulthood). To address this, using single-cell RNA sequencing, we analyzed testis cell composition and hormone receptor expression changes during three important developmental stages of Hu sheep. We observed significant changes in the composition of somatic and germ cells in different Hu sheep testis developmental stages. Furthermore, we analyzed the FSHR, LHR and AR distribution and expression changes at three important periods and verified them by qRT-PCR and immunofluorescence. Our results suggest that after birth, the proportion of germ cells increased gradually, peaking in adulthood; the proportion of Sertoli cells decreased gradually, reaching the lowest in adulthood; and the proportion of Leydig cells increased and then decreased, reaching the lowest in adulthood. In addition, FSHR, LHR and AR are mainly located in Sertoli, Leydig and germ cells. LHR and FSHR expression decreased with increasing age, while AR expression increased and then decreased with increasing age.

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