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Rapid detection of aflatoxin B-1 on membrane by dot-immunogold filtration assay

文献类型： 外文期刊

作者： Ye, Yang 1 ; Zhou, Youxiang 4 ; Mo, Zhenzhen 1 ; Cheng, Wei 1 ; Yang, Sha 1 ; Wang, Xiaohong 1 ; Chen, Fusheng 1 ;

作者机构： 1.Huazhong Agr Univ, Coll Food Sci & Technol, Wuhan 430070, Hubei Province, Peoples R China

2.Huazhong Agr Univ, Minist Agr, Key Lab Food Safety Evaluat, Wuhan 430070, Hubei Province, Peoples R China

3.Huazhong Agr Univ, Natl Key Lab Agromicrobiol, Wuhan 430070, Hubei Province, Peoples R China

4.Minist Agr, Food Qual Inspect & Testing Ctr, Wuhan 430064, Hubei Province, Peoples R China

关键词： DIGFA;Colloidal gold;Mycotoxin;AFB(1);Monoclonal antibody

期刊名称：TALANTA （ 影响因子：6.057； 五年影响因子：5.386 ）

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收录情况： SCI

摘要： Immunofiltration assay for mycotoxins in which nitrocellulose membrane (NCM) was used as a support and enzyme was used as the label has been developed since the late 1980s. As colloidal gold is a good labeling substance that can accelerate antibody-antigen reaction which result can be read directly by naked eyes, the colloidal gold particles could replace the enzyme to be labeled to antibody in aflatoxin B-1 (AFB(1)) immunoassay. Dot-immunogold filtration assay (DIGFA) of AFB(1) on NCM was developed in this study. At first, the colloidal gold was synthesized and colloidal gold-monoclonal antibody (McAb) conjugates against AFB(1) were prepared at pH 7.0 of colloidal gold solution, 0.018 mg/mL of McAb. Then the colloidal gold-McAb conjugates were used to develop AFB(1) DIGFA, which detection time was only 15 min, six times less than that of ELISA. With this method to determine the standard AFB(1) solution, the results demonstrated a visual detection limit of approximately 2 ng/mL of AFB(1). which was similar to that of ELISA. This method had good specificities for AFG(1), AFG(2) and AFM(1) and a little cross-reactivity with AFB(2). 45 food samples collected from the markets were subjected to DIGFA and the results showed that one corn sample was positive and in agreement that of HPLC. It is suggested that DIGFA developed in current study has a potential use as a rapid and cost-effective screening tool for the determination of AFB(1) in foods in the field within 15 min without complicated steps.

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