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Loop-Mediated Isothermal Amplification for Rapid Detection of Mating Types of Villosiclava virens

文献类型： 外文期刊

作者： Pan, Xiayan 1 ; Wang, Xiao 1 ; Yu, Junjie 1 ; Yu, Mina 1 ; Cao, Huijuan 1 ; Yong, Mingli 1 ; Song, Tianqiao 1 ; Qi, Zhongqiang 1 ; Du, Yan 1 ; Zhang, Rongsheng 1 ; Liu, Yongfeng 1 ;

作者机构： 1.Jiangsu Acad Agr Sci, Inst Plant Protect, Nanjing 210014, Jiangsu, Peoples R China

2.Nanjing Agr Univ, Coll Plant Protect, Nanjing 210095, Jiangsu, Peoples R China

关键词： loop-mediated isothermal amplification; mating type; sexual reproduction; Villosiclava virens

期刊名称：PLANT DISEASE （ 影响因子：4.614； 五年影响因子：5.33 ）

ISSN： 0191-2917

年卷期： 2022 年 106 卷 4 期

页码：

收录情况： SCI

摘要： Rice false smut (RFS), caused by Villosiclava virens, is an important fungal disease in panicles of rice. V. virens is a heterothallic ascomycete controlled by two opposite idiomorphs, MAT1-1 and MAT1-2. Previous study showed that sexual reproduction of V. virens plays an important role in the epidemic of RFS. In this study, we developed a loop-mediated isothermal amplification (LAMP) assay to detect the mating type of V. virens easily and rapidly by using specific primers based on the mating type genes MAT1-1-2 and MAT1-2-1, respectively. The LAMP assay used only a water/dry bath and could recognize the mating type of V. virens in just 45 min. The LAMP assay was so sensitive that it could detect small amounts of V. virens genomic DNA (as low as 2.0 pg of MAT1-1 and 200.0 pg of MAT1-2) and was 10 times more sensitive than PCR. In addition, we demonstrated the application of mating type via LAMP assay by assessing the genomic DNA of V. virens isolated from rice fields. The high efficiency and specificity of this LAMP assay suggest that it can be used as a rapid testing tool in mating type recognition of V. virens isolates in the field.

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