A fluorescent lateral flow immunoassay based on CdSe/CdS/ZnS quantum dots for sensitive detection of olaquindox in feedstuff
文献类型: 外文期刊
作者: Hu, Mei 1 ; Hu, Xiaofei 2 ; Wang, Guoqiang 3 ; Cheng, Yongxia 1 ; Yu, Xiaoling 1 ; Huang, Xianqing 1 ; Li, Yu 1 ;
作者机构: 1.Henan Agr Univ, Coll Food Sci & Technol, Zhengzhou 450002, Henan, Peoples R China
2.Henan Acad Agr Sci, Key Lab Anim Immunol, Minist Agr, Zhengzhou 450002, Henan, Peoples R China
3.Henan Univ Chinese Med, Acad Chinese Med Sci, Zhengzhou 450002, Henan, Peoples R China
关键词: Olaquindox detection; CdSe; CdS; ZnS quantum dots; Lateral flow immunoassay
期刊名称:FOOD CHEMISTRY ( 影响因子:8.8; 五年影响因子:8.6 )
ISSN: 0308-8146
年卷期: 2023 年 419 卷
页码:
收录情况: SCI
摘要: A portable fluorescence immunosensor based on the CdSe/CdS/ZnS quantum dots (QDs) with multiple-shell structure was fabricated for the precise quantification of olaquindox (OLA). The QDs labeled anti-OLA anti-body used as bioprobe played an important role in the design and preparation of a lateral flow test strip. Due to the strong fluorescent intensity of QDs, the sensitivity is greatly improved. The quantitative results were obtained using a fluorescent strip scan reader within 8 min, and the calculated limit of detection for OLA at 0.12 mu g/kg, which was 2.7 times more sensitive than that of the conventional colloidal gold-based strips method. Acceptable recovery of 85.0%-95.5% was obtained by the spiked samples. This newly established QDs-based strip immu-noassay method is suitable for the on-site detection and rapid initial screening of OLA in swine feedstuff, and is potentially applied for the detection of other veterinary drugs to ensure food safety.
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