您好，欢迎访问中国水产科学研究院 机构知识库！
中国水产科学研究院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Molecular cloning, characterization and mRNA expression of two antibacterial peptides: Crustin and anti-lipopolysaccharide factor in swimming crab Portunus trituberculatus

文献类型： 外文期刊

作者： Yue, Feng 1 ; Pan, Luqing 1 ; Miao, Jingjing 1 ; Zhang, Lin 1 ; Li, Jian 2 ;

作者机构： 1.Ocean Univ China, Minist Educ, Key Lab Mariculture, Qingdao 266003, Peoples R China

2.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Qingdao 266003, Peoples R China

关键词： Crustin;Anti-lipopolysaccharide factor;cDNA cloning

期刊名称：COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY （ 影响因子：2.231； 五年影响因子：2.215 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： In this study, the cDNAs of a crustin and an anti-lipopolysaccharide factor (ALF) were cloned from the hemocytes of swimming crab Portunus trituberculatus using rapid amplification of cDNA ends (RACE) approaches. The full-length cDNA of P. trituberculatus crustin (denoted as PtCrustin) was of 584 bp, including an open reading frame (ORF) of 333 bp encoding a 21 amino acids signal peptide and a mature polypeptide of 89 amino acids with the predicted molecular mass of 10.08 kDa. The typical conserved cysteine residues containing WAP domain at the C-terminal displayed high similarity to other crustins. Moreover, the full-length cDNA of P. trituberculatus ALF (denoted as PtALF) was of 1050 bp, including an ORF of 372 bp encoding a 26 amino acids signal peptide and a mature polypeptide of 97 amino acids with the predicted molecular mass of 11.35 kDa. PtALF contained two conserved cysteine residues and showed high similarity to other crab ALFs. Using fluorescent real-time quantitative PCR. PtCrustin mRNA was expressed at the highest level in hemocytes and higher level in stomach while extremely low in hepatopancreas, muscle, gill and heart. In contrast, the PtALF mRNA expression was observed in all tissues. The relative expression levels of PtCrustin and PtALF in hemocytes of P. trituberculatus showed a clear time-dependent response after challenge with Vibrio alginolyticus. These results indicate that PtCrustin and PtALF are two acute-phase proteins involved in the immune responses of P. trituberculatus.

  • 相关文献

[1]Immune responses and expression of immune-related genes in swimming crab Portunus trituberculatus exposed to elevated ambient ammonia-N stress. Yue, Feng,Pan, Luqing,Xie, Peng,Zheng, Debin,Li, Jian.

[2]A novel anti-lipopolysaccharide factor SpALF6 in mud crab Scylla paramamosain exhibiting different antimicrobial activity from its single amino acid mutant. Hou, Zhi-Guo,Wang, Yuan,Fang, Wen-Hong,Zhao, Shu,Zhang, Jing-Xiao,Li, Xin-Cang,Hou, Zhi-Guo,Hui, Kaimin,Ma, Hongyu.

[3]Three different anti-lipopolysaccharide factors identified from giant freshwater prawn, Macrobrachium rosenbergii. Ren, Qian,Jie-Du,Hui, Kai-Min,Wang, Wen,Ren, Qian,Jie-Du,Hui, Kai-Min,Wang, Wen,Zhang, Zhao,Zhang, Chi-Yu,Li, Xin-chang. 2012

[4]Response of a Mu-class glutathione S-transferase from black tiger shrimp Penaeus monodon to aflatoxin B1 exposure. Wang, Yun,Huang, Jianhua,Duan, Yafei,Wang, Jun,Fu, Mingjun,Lin, Heizhao,Liu, Lihui. 2016

[5]Polygenic expression of somatostatin in orange-spotted grouper (Epinephelus coioides): Molecular cloning and distribution of the mRNAs encoding three somatostatin precursors. Ying, Y,Li, WS,Lin, HR. 2005

[6]Identification and characterization of a Macrobrachium nipponense ferritin subunit regulated by iron ion and pathogen challenge. Sun, Shengming,Gu, Zhimin,Gu, Zhimin,Sun, Shengming,Fu, Hongtuo,Zhu, Jian,Ge, Xianping,Xuan, Fujun.

[7]Molecular Characterization and Expression Analyses of the Complement Component C8 alpha, C8 beta and C9 Genes in Yellow Catfish (Pelteobagrus fulvidraco) after the Aeromonas hydrophila Challenge. Zheng, Huan,Ji, Wei,Zhang, Gui-Rong,Zhang, Xiao-Ting,Wei, Kai-Jian,Yang, Rui-Bin,Gardner, Jonathan P. A.,Zheng, Huan,Ji, Wei,Zhang, Gui-Rong,Zhang, Xiao-Ting,Shi, Ze-Chao,Wei, Kai-Jian,Yang, Rui-Bin,Gardner, Jonathan P. A.,Shi, Ze-Chao,Gardner, Jonathan P. A.. 2016

[8]cDNA structure, genomic organization and expression patterns of visfatin in silver Prussian carp (Carassius auratus gibelio). Dong Xu,Zou Shiping,Wu Xiaoxiong,Dai Hanchuan,Zeng Cuiping,Wang Xu,Long Liangqi,Zou Shiping. 2011

[9]Characteristics of Cyclin B and its potential role in regulating oogenesis in the red claw crayfish (Cherax quadricarinatus). Wang, L. M.,Dong, Z. J.,Wang, L. M.,Lv, W. W.,Zuo, D.,Zhao, Y. L.. 2015

[10]cDNA cloning and mRNA expression of the translationally controlled tumor protein (TCTP) gene from Japanese sea perch (Lateolabrax japonicus). Qiu, LH,Song, LS,Wu, LT,Xu, W,Jiang, SG.

[11]The two channel catfish intelectin genes exhibit highly differential patterns of tissue expression and regulation after infection with Edwardsiella ictaluri. Takano, Tomokazu,Sha, Zhenxia,Peatman, Eric,Terhune, Jeffery,Li, Hong,Kucuktas, Huseyin,Li, Ping,Liu, Zhanjiang,Sha, Zhenxia,Edholm, Eva-Stina,Wilson, Melanie.

[12]Characterization of -catenin 1 during the gonad development in the common carp (Cyprinus carpio). Wang, Lan-mei,Zhu, Wen-bin,Fu, Jian-jun,Dong, Zai-jie,Song, Fei-biao,Dong, Juan-juan,Dong, Zai-jie.

[13]Molecular characterization of Kiss2 receptor and in vitro effects of Kiss2 on reproduction-related gene expression in the hypothalamus of half smooth tongue sole (Cynoglossus semilaevis). Wang, Bin,Liu, Quan,Liu, Xuezhou,Xu, Yongjiang,Shi, Bao,Wang, Bin,Liu, Xuezhou,Xu, Yongjiang,Shi, Bao,Liu, Quan.

[14]Molecular characterization and expression analysis of three TLR genes in yellow catfish (Pelteobagrus fulvidraco): Responses to stimulation of Aeromonas hydrophila and TLR ligands. Wang, Kai-Lun,Ji, Wei,Zhang, Gui-Rong,Wei, Kai-Jian,Zhang, Xiao-Ting,Zheng, Huan,Fan, Qi-Xue,Wang, Kai-Lun,Ji, Wei,Zhang, Gui-Rong,Wei, Kai-Jian,Shi, Ze-Chao,Zhang, Xiao-Ting,Zheng, Huan,Fan, Qi-Xue,Shi, Ze-Chao.

作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：中国水产科学研究院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台