Integrated Analysis of Transcriptome and Metabolome Reveals Differential Responses to Alternaria brassicicola Infection in Cabbage (Brassica oleracea var. capitata)
文献类型： 外文期刊
作者： Lei, Jinzhou 1 ; Zhang, Wei 2 ; Yu, Fangwei 2 ; Ni, Meng 2 ; Liu, Zhigang 1 ; Wang, Cheng 3 ; Li, Jianbin 2 ; Song, Jianghua 1 ; Wang, Shenyun 1 ;
作者机构： 1.Anhui Agr Univ, Coll Hort, Anhui Prov Key Lab Hort Crop Qual Biol, Hefei 230036, Peoples R China
2.Jiangsu Acad Agr Sci, Inst Vegetable Crops, Jiangsu Key Lab Hort Crop Genet Improvement, Nanjing 210014, Peoples R China
3.Hubei Engn Univ, Coll Life Sci & Technol, Key Lab Qual Control Characterist Fruits & Vegetab, Xiaogan 432000, Peoples R China
关键词： cabbage; Alternaria brassicicola; reactive oxygen species; jasmonic acid signaling; indolic glucosinolate biosynthesis; transcriptome; metabolome
期刊名称：GENES （ 影响因子：3.5； 五年影响因子：3.9 ）
ISSN：
年卷期： 2024 年 15 卷 5 期
页码：
收录情况： SCI
摘要： Black spot, caused by Alternaria brassicicola (Ab), poses a serious threat to crucifer production, and knowledge of how plants respond to Ab infection is essential for black spot management. In the current study, combined transcriptomic and metabolic analysis was employed to investigate the response to Ab infection in two cabbage (Brassica oleracea var. capitata) genotypes, Bo257 (resistant to Ab) and Bo190 (susceptible to Ab). A total of 1100 and 7490 differentially expressed genes were identified in Bo257 (R_mock vs. R_Ab) and Bo190 (S_mock vs. S_Ab), respectively. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis revealed that "metabolic pathways", "biosynthesis of secondary metabolites", and "glucosinolate biosynthesis" were the top three enriched KEGG pathways in Bo257, while "metabolic pathways", "biosynthesis of secondary metabolites", and "carbon metabolism" were the top three enriched KEGG pathways in Bo190. Further analysis showed that genes involved in extracellular reactive oxygen species (ROS) production, jasmonic acid signaling pathway, and indolic glucosinolate biosynthesis pathway were differentially expressed in response to Ab infection. Notably, when infected with Ab, genes involved in extracellular ROS production were largely unchanged in Bo257, whereas most of these genes were upregulated in Bo190. Metabolic profiling revealed 24 and 56 differentially accumulated metabolites in Bo257 and Bo190, respectively, with the majority being primary metabolites. Further analysis revealed that dramatic accumulation of succinate was observed in Bo257 and Bo190, which may provide energy for resistance responses against Ab infection via the tricarboxylic acid cycle pathway. Collectively, this study provides comprehensive insights into the Ab-cabbage interactions and helps uncover targets for breeding Ab-resistant varieties in cabbage.
- 相关文献
作者其他论文 更多>>
-
Construction of a high-density genetic map for yardlong bean and identification of ANT1 as a regulator of anthocyanin biosynthesis
作者：Zhang, Hongmei;Zhang, Wei;Liu, Xiaoqing;Chen, Xin;Chen, Huatao;Meng, Shan;Yan, Wei;Hui, Linchong;Chen, Wei
关键词：
-
Homoeologous exchanges contribute to branch angle variations in rapeseed: Insights from transcriptome, QTL-seq and gene functional analysis
作者：Sun, Chengming;Zhou, Xiaoying;Fu, Sanxiong;Wang, Xiaodong;Peng, Qi;Gao, Jianqin;Chen, Feng;Zhang, Wei;Hu, Maolong;Zhang, Jiefu;Wu, Jian;Liu, Huimin;Wang, Youping;Xue, Zhifei;Fu, Tingdong;Yi, Bin
关键词：Rapeseed; Branch angle; Homoeologous exchange; WGCNA; QTL-seq; WRKY40
-
A genome-wide association analysis for salt tolerance during the soybean germination stage and development of KASP markers
作者：Wang, Junyan;Zhou, Miaomiao;Su, Chengfu;Wang, Junyan;Zhou, Miaomiao;Zhang, Hongmei;Liu, Xiaoqing;Zhang, Wei;Wang, Qiong;Jia, Qianru;Chen, Huatao;Xu, Donghe;Chen, Huatao
关键词：soybean; salt tolerance; germination stage; genome-wide association analysis; KASP marker
-
Therapeutic efficacy of a K5-specific phage and depolymerase against Klebsiella pneumoniae in a mouse model of infection
作者：Li, Pei;Zheng, Xiangkuan;Xu, Sixiang;Zhou, Yu;Qin, Xiayan;Hu, Zimeng;Ma, Jiale;Zhang, Wei;Li, Pei;Zheng, Xiangkuan;Xu, Sixiang;Zhou, Yu;Hu, Zimeng;Zhang, Wei;Guo, Genglin;Yu, Yanfei;Tan, Zhongming;Chen, Long
关键词：Klebsiella pneumoniae; phage; depolymerase; K5; hypervirulent; capsule
-
Selected Electrosynthesis of 3-Aminopyrazoles from α,β-Alkynic Hydrazones and Secondary Amines
作者：Tang, Dong;Zhang, Wei;Ji, Jingwen;Jiang, Rui;Wan, Yaya;Zhou, Pengjuan;Ma, Wei
关键词：
-
Ultra-Highly Stiff and Tough Shape Memory Polyurea with Unprecedented Energy Density by Precise Slight Cross-Linking
作者：Chen, Jiaoyang;Yao, Bowen;Geng, Yuhao;Xu, Jianhua;Chen, Tao;Jing, Jiajie;Fu, Jiajun;Wang, Zhifeng;Wang, Cheng
关键词：covalent and supramolecular bonds; environmental stability and recyclability; high energy density; mechanical robustness and toughness; shape memory polymers
-
Genome-Wide Association Study on Candidate Genes Associated with Soybean Stem Pubescence and Hilum Colors
作者：Zhou, Miaomiao;Wang, Junyan;Su, Chengfu;Chen, Huatao;Wang, Qiong;Chen, Huatao;Hu, Shengyan;Xiong, Yawen;Chen, Huatao;Jia, Qianru;Zhang, Hongmei;Zhang, Wei;Wang, Qiong
关键词：soybean; stem pubescence color; hilum color; genome-wide association study; SNP; candidate gene
