文献类型： 外文期刊

作者： Bi, Shao-Shuai ¹ ; Talukder, Milton ¹ ; Jin, Hai-Tao ⁴ ; Lv, Mei-Wei ¹ ; Ge, Jing ¹ ; Zhang, Cong ¹ ; Li, Jin-Long ¹ ;

作者机构： 1.Northeast Agr Univ, Coll Vet Med, Harbin 150030, Peoples R China

2.West Anhui Univ, Coll Biotechnol & Pharmaceut Engn, Luan 237012, Peoples R China

3.Patuakhali Sci & Technol Univ, Fac Anim Sci & Vet Med, Dept Physiol & Pharmacol, Barishal 8210, Bangladesh

4.Heilongjiang Acad Agr Sci, Qual & Safety Inst Agr Prod, Harbin 150010, Peoples R China

5.Henan Agr Univ, Coll Vet Med, Zhengzhou 450002, Peoples R China

6.Northeast Agr Univ, Prov Educ Dept Heilongjiang Common Anim Dis Preven, Key Lab, Harbin 150030, Peoples R China

7.Northeast Agr Univ, Heilongjiang Key Lab Lab Anim & Comparat Med, Harbin 150030, Peoples R China

关键词： Nano-selenium; Cadmium; Selenoprotein transcriptome; MTF1-mediated metal response; Cerebellar injury

期刊名称：ANIMAL NUTRITION （ 影响因子：5.285； 五年影响因子：7.059 ）

ISSN： 2405-6383

年卷期： 2022 年 11 卷

页码：

收录情况： SCI

摘要： This study aims to investigate the role of metal regulatory transcription factor 1 (MTF1)-mediated metal response in cadmium (Cd)-induced cerebellar injury, and to evaluate the antagonistic effects of nano-selenium (Nano-Se) against Cd toxicity. A total of 80 chicks (1 d old, male, Hy-Line Variety White) were randomly allocated to 4 treatment groups for 3 months: the control group (fed with a basic diet, n = 20), the Nano-Se group (basic diet with 1 mg/kg nano-Se 1 mg/kg Nano-Se in basic diet, n = 20), the Nano-Se thorn Cd group (basic diet with 1 mg/kg Nano-Se and 140 mg/kg CdCl2, n = 20) and the Cd group (basic diet with 140 mg/kg CdCl2 , n = 20). The results of the experiment showed that the Purkinje cells were significantly decreased with their degradation and indistinct nucleoli after Cd exposure. Moreover, expo-sure to Cd caused a significant accumulation of Cd and cupper. However, the contents of Se, iron, and zinc were decreased, thereby disturbing the metal homeostasis in the cerebellum. The Cd exposure also resulted in high levels of malondialdehyde (MDA) and down regulation of selenoprotein transcriptome. Furthermore, the expressions of MTF1, metallothionein 1 (MT1), MT2, zinc transporter 3 (ZNT3), ZNT5, ZNT10, zrt, irt-like protein 8 (ZIP8), ZIP10, transferrin (TF), ferroportin 1 (FPN1), ATPase copper transporting beta (ATP7B), and copper uptake protein 1 (CTR1) were inhibited by Cd exposure. However, all these changes were significantly alleviated by the supplementation of Nano-Se. This study proved that Cd could disorder metal homeostasis and induce oxidative stress, whereas Nano-Se could relieve all these negative effects caused by Cd via activating the MTF1-mediated metal response in the cerebellum of chicken.