Determination of Streptomycin Residue in Cucumber and Chinese Cabbage by High-Performance Liquid Chromatography with Postcolumn Derivatization and Fluorometric Detection
文献类型： 外文期刊
作者： Chen, Benjing 1 ; Zhang, Hongyan 1 ; Lin, Baoxiang 3 ; Ge, Jing 1 ; Qiu, Lihong 1 ;
作者机构： 1.China Agr Univ, Coll Sci, Beijing 100193, Peoples R China
2.Shanghai Acad Agr Sci, Inst Agrofood Stand & Testing Technol, Shanghai 201403, Peoples R China
3.Inst Hort Res, Heilongjiang Acad Agr Sci, Harbin 150069, Peoples R China
期刊名称：JOURNAL OF AOAC INTERNATIONAL （ 影响因子：1.913； 五年影响因子：1.754 ）
ISSN： 1060-3271
年卷期： 2012 年 95 卷 2 期
页码：
收录情况： SCI
摘要： A sensitive and accurate method was developed for the determination of streptomycin using HPLC followed by postcolumn derivatization and fluorometric detection. The analyte was extracted, using aqueous solution from cucumber and Chinese cabbage, by a two-step SPE procedure. The extraction, cleanup, and chromatography conditions were optimized, and the performance of the analysis method was evaluated. The conditions of chromatography were as follows: the separation was performed on a C18 column; the isocratic mobile phase consisted of acetonitrile and a mixed solution containing 10 mM sodium 1,2-naphthoquinone-4-sulfonate and 0.4 mM sodium 1-heptanesulfonate (25+75, v/v); and the flow rate was 1 mL/min. The fluorescence detector was set at an excitation wavelength of 263 nm and an emission wavelength of 435 nm. The calibration curve was linear over the range of 50-2000 ng/mL, with a correlation coefficient of 0.9995. The LOD and LOQ were 10 and 30 ng/g, respectively, in both cucumber and Chinese cabbage. The method was validated for selectivity, linearity, precision, and accuracy. The intraday and interday precision and accuracy were within 10%. The mean recoveries from spiked samples were more than 75%, with RSD lower than 10%.
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