Molecular identification and function characterization of four alternative splice variants of trim25 in Japanese flounder (Paralichthys olivaceus)
文献类型： 外文期刊
作者： Guo, Yanan 1 ; Sun, Zhaodi 1 ; Zhang, Yitong 1 ; Wang, Guixing 1 ; He, Zhongwei 1 ; Liu, Yufeng 1 ; Ren, Yuqin 1 ; Wang, Yufen 1 ; Fu, Yuanshuai 2 ; Hou, Jilun 1 ;
作者机构： 1.Chinese Acad Fishery Sci, Beidaihe Cent Expt Stn, Hebei Key Lab Bohai Sea Fish Germplasm Resources, Qinhuangdao 066100, Hebei, Peoples R China
2.Minist Agr, Key Lab Freshwater Aquat Genet Resources, Shanghai 201306, Peoples R China
3.Shanghai Collaborat Innovat Aquat Anim Genet & Br, Shanghai 201306, Peoples R China
4.Shanghai Ocean Univ, Lab Cell & Mol Biol, Shanghai 201306, Peoples R China
关键词： Alternative splicing; Japanese flounder; trim25; Innate immune response
期刊名称：FISH & SHELLFISH IMMUNOLOGY （ 影响因子：4.622； 五年影响因子：4.799 ）
ISSN： 1050-4648
年卷期： 2022 年 120 卷
页码：
收录情况： SCI
摘要： Trim25 is a member of Tripartite Motif (TRIM) family. Previous studies report that trim25 modulates antiviral activity by activating RIG-I. In this study we explored the four alternative splicing (AS) variants X1-X4 of Japanese flounder trim25. The sequences of the AS variants were highly conserved. Expression levels of trim25 X1-X4 were increased after 12 h of poly I:C treatment in vitro. In vivo expression of X2-X4 in liver, kidney (except X2) and blood was significantly up-regulated in early stages of poly I:C treatment. Subcellular localization analysis showed that Trim25 X1-X4 were distributed in different cellular organelles. The recombinant vector pcDNA3.1Trim25 X1-X4 were successfully overexpressed in Flounder cells and the samples were collected. Expression patterns of RIG-I pathway genes dhx58, traf6, traf2, nfkbia and il-8 were explored in vitro and in vivo after poly I:C treatment, as well as overexpressed samples. The findings of this study imply that AS variants of trim25 confer antiviral activity in Japanese flounder by modulating innate immune response.
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