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Dietary Morus alba L. leaf supplementation improves hepatic lipid accumulation of laying hens via downregulating CircACACA

文献类型： 外文期刊

作者： Chen, Fang 1 ; Huang, Jing 1 ; Zhao, Na 1 ; Jin, Feng 1 ; Fan, Qiweng 1 ; Du, Encun 1 ; Wei, Jintao 1 ;

作者机构： 1.Hubei Acad Agr Sci, Inst Anim Husb & Vet Sci, Hubei Key Lab Anim Embryo & Mol Breeding, Wuhan 430064, Peoples R China

2.Key Lab Prevent & Control Agents Anim Bacteriosis, Wuhan 430064, Peoples R China

关键词： hen; mulberry leaf; fatty liver; lipid metabolism; CircRNA

期刊名称：POULTRY SCIENCE （ 影响因子：4.4； 五年影响因子：4.4 ）

ISSN： 0032-5791

年卷期： 2023 年 102 卷 11 期

页码：

收录情况： SCI

摘要： Fatty liver hemorrhagic syndrome (FLHS) is the most common metabolic disease in laying hens. Morus alba L. (mulberry) leaf has the effect of regu-lating lipid metabolism. We evaluated the effects of die-tary 3% mulberry leaf (MUL) supplementation in production performance, egg quality, and liver lipid depo-sition in laying hens. Differentially expressed genes and circRNAs in the liver were identified using whole-tran-scriptome sequencing. We also evaluated the effects of the MUL extract using an in vitro model of primary hepato-cytes induced by free fatty acids and explored the role of key circRNAs in this process. Dietary supplementation with 3% MUL alleviated liver steatosis in laying hens, as shown by decreased fatty liver color score, relative liver weight (P < 0.01), and triglyceride levels (P < 0.05), and showed a tendency to reduce the mortality rate of laying hens (P = 0.09). In addition, mulberry leaf supplementa-tion significantly reduced cholesterol content in egg yolk (P < 0.01). Dietary mulberry leaf supplementation down-regulated the expression of genes involved in fatty acid and cholesterol biosynthesis, and upregulated the expres-sion of fatty acid oxidation-related genes in the liver. Cir-cACACA, which is derived from exons 2 and 3 of the acetyl-CoA carboxylase alpha (ACACA) pre-mRNA, was significantly reduced in the MUL group (P < 0.01). Upregulation of circACACA expression reversed the lipid-lowering effect of mulberry leaf extract by upregulat-ing sterol regulatory element-binding proteins 1 c (SREBP-1c) and fatty acid synthase (FASN) (P < 0.05). Overall, mulberry leaf is an effective therapeutic strategy for FLHS in hens and can improve liver lipid metabolism by downregulating circACACA.

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