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Efficient CRISPR/Cas9-mediated genome editing in sheepgrass (Leymus chinensis)

文献类型： 外文期刊

作者： Lin, Zhelong 1 ; Chen, Lei 1 ; Tang, Shanjie 1 ; Zhao, Mengjie 1 ; Li, Tong 1 ; You, Jia 3 ; You, Changqing 1 ; Li, Boshu 4 ; Zhao, Qinghua 1 ; Zhang, Dongmei 3 ; Wang, Jianli 3 ; Shen, Zhongbao 3 ; Song, Xianwei 1 ; Zhang, Shuaibin 1 ; Cao, Xiaofeng 1 ;

作者机构： 1.Chinese Acad Sci, State Key Lab Plant Genom, Beijing 100101, Peoples R China

2.Chinese Acad Sci, Inst Genet & Dev Biol, Natl Ctr Plant Gene Res, Beijing 100101, Peoples R China

3.Heilongjiang Acad Agr Sci, Inst Forage & Grassland Sci, Harbin 150086, Peoples R China

4.Chinese Acad Sci, Ctr Genome Editing, Inst Genet & Dev Biol, State Key Lab Plant Cell & Chromosome Engn, Beijing 100101, Peoples R China

关键词： Agrobacterium; genetic transformation; genome editing; LcTB1; sheepgrass

期刊名称：JOURNAL OF INTEGRATIVE PLANT BIOLOGY （ 影响因子：11.4； 五年影响因子：10.1 ）

ISSN： 1672-9072

年卷期： 2023 年 65 卷 11 期

页码：

收录情况： SCI

摘要： The lack of genome editing platforms has hampered efforts to study and improve forage crops that can be grown on lands not suited to other crops. Here, we established efficient Agrobacterium-mediated clustered regularly interspaced palindromic repeats (CRISPR)/CRISPR-associated nuclease 9 (Cas9) genome editing in a perennial, stress-tolerant forage grass, sheepgrass (Leymus chinensis). By screening for active single-guide RNAs (sgRNAs), accessions that regenerate well, suitable Agrobacterium strains, and optimal culture media, and co-expressing the morphogenic factor TaWOX5, we achieved 11% transformation and 5.83% editing efficiency in sheepgrass. Knocking out Teosinte Branched1 (TB1) significantly increased tiller number and biomass. This study opens avenues for studying gene function and breeding in sheepgrass.

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