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Pluripotency and chimera competence of an embryonic stem cell line from the sea perch (Lateolabrax japonicus)

文献类型: 外文期刊

作者: Chen, Song-Lin 1 ; Sha, Zhen-Xia 2 ; Ye, Han-Qing 2 ; Liu, Yang 2 ; Tian, Yong-Sheng 3 ; Hong, Yunhan; Tang, Qi-She 1 ;

作者机构: 1.Chinese Acad Fisheries Sci, Yellow Sea fisheries Res Inst, Key Lab Sustainable Utilizat Marine Fisheries Res, Minist Agr, Qingdao 266071, Peoples R China

2.Chinese Acad Fisheries Sci, Yellow Sea fisheries Res Inst, Key Lab Sustainable Utilizat Marine Fisheries Res, Minist Agr, Qingdao 266071, Peoples R China; Natl Univ Singapore, Dept Biol Sci, Singapore 119260, Singapore

3.Chinese Acad Fisheries Sci, Yellow Sea fisheries Res Inst, Key Lab Sustainable Utilizat Marine Fisheries Res, Minist Agr, Qingdao 266071, Peoples R China; Natl Univ Singapore, Dept Biol Sci, Singapore 119260,

关键词: Animals;Cell Differentiation physiology;Cell Line;Chimera physiology;Embryo;Nonmammalian cytology physiology;Embryonic Stem Cells cytology physiology;Green Fluorescent Proteins biosynthesis genetics;Microscopy;Fluorescence;Perches embryology physiology;Transfection veterinary;Zebrafish embryology

期刊名称:MARINE BIOTECHNOLOGY ( 影响因子:3.619; 五年影响因子:3.322 )

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收录情况: SCI

摘要: A stable GFP-expressing (GFP(+)LJES1) cell strain was developed from the LJES1 cells obtained from sea perch (Lateolabrax japonicus,) embryos. GFP(+)LJES1 cells were induced in vitro by RA to differentiate into a variety of cell types and also had the ability to form embryoid body-like structures in suspension culture. To determine the differentiation potential of LJES1 cells in vivo, GFP(+)LJES1 cells were transplanted into sea perch and zebrafish embryos at mid-blastula stage. Twenty out of 478 transplanted sea perch embryos contained GFP-expressing LJES1 cells 24 h after microinjection. Fifteen chimera embryos developed into fry. In these chimeras, the GFP(+)LJES1 cells contributed to a variety of tissues including the head and trunk. In zebrafish, 221 embryos were microinjected with GFP(+)LJES1 cells and 22 chimera embryos and fries expressing GFP were obtained. Donor GFP(+)LJES1 cells contributed to various tissues in head and trunk of zebrafish embryos and hatched fry.

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