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Cloning, characterization, and transformation of the phosphoethanolamine N-methyltransferase gene (ZmPEAMT1) in maize (Zea mays L.)

文献类型： 外文期刊

作者： Wu, Suowei 1 ; Yu, Zhanwang 2 ; Wang, Fengge 2 ; Li, Weihua 2 ; Ye, Chunjiang 3 ; Li, Jun; Tang, Jihua; Ding, Junqi 1 ;

作者机构： 1.Chinese Acad Sci, State Key Lab Plant Genom, Inst Genet & Dev Biol, Beijing 100101, Peoples R China

2.Chinese Acad Sci, State Key Lab Plant Genom, Inst Genet & Dev Biol, Beijing 100101, Peoples R China; Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China; Beijing Acad Agr & Forestry Sci, Maize Res Ctr, Beijing 100089, Peoples R China; Henan Agr Univ, Coll Agron, Zhengzhou 450002, Peoples R China

3.Chine

关键词： Maize (Zea mays L.);Phosphoethanolamine N-methyltransferase;Promoter;TAIL-PCR;Transgenic plants

期刊名称：MOLECULAR BIOTECHNOLOGY （ 影响因子：2.695； 五年影响因子：2.303 ）

ISSN：

年卷期：

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收录情况： SCI

摘要： N-methylation of phosphoethanolamine, the committing step in choline (Cho) biosynthesis in plants, is catalyzed by S-adenosyl-l-methionine: phosphoethanolamine N-methyltransferase (PEAMT, EC 2.1.1.103). Herein we report the cloning and characterization of the novel maize phosphoethanolamine N-methyltransferase gene (ZmPEAMT1) using a combination of bioinformatics and a PCR-based allele mining strategy. The cDNA sequence of ZmPEAMT1 gene is 1,806 bp in length and translates a 495 amino acids peptide. The upstream promoter sequence of ZmPEAMT1 were obtained by TAIL-PCR, and contained four kinds of putative cis-acting regulatory elements, including stress-responsive elements, phytohormone-responsive elements, pollen developmental special activation elements, and light-induced signal transduction elements, as well as several other structural features in common with the promoter of rice and Arabidopsis homologies. RT-PCR analysis showed that expression of ZmPEAMT1 was induced by salt stress and suppressed by high temperature. Over-expression of ZmPEAMT1 enhanced the salt tolerance, root length, and silique number in transgenic Arabidopsis. These data indicated that ZmPEAMT1 maybe involved in maize root development and stress resistance, and maybe having a potential application in maize genetic engineering.

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