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Fine mapping of an adult-plant resistance gene to powdery mildew in soybean cultivar Zhonghuang 24

文献类型： 外文期刊

作者： Zhou, Qian 1 ; Jiang, Bingzhi 1 ; Cheng, Yanbo 1 ; Ma, Qibin 1 ; Xia, Qiuju 5 ; Jiang, Ze 1 ; Cai, Zhandong 1 ; Nian, Hai 1 ;

作者机构： 1.South China Agr Univ, State Key Lab Conservat & Utilizat Subtrop Agrobio, Guangzhou 510642, Guangdong, Peoples R China

2.South China Agr Univ, Guangdong Prov Lab Lingnan Modern Agr Sci & Techno, Guangzhou 510642, Guangdong, Peoples R China

3.South China Agr Univ, Coll Agr, Key Lab Plant Mol Breeding Guangdong Prov, Guangzhou 510642, Guangdong, Peoples R China

4.Guangdong Acad Agr Sci, Crops Res Inst, Guangdong Prov Key Lab Crops Genet & Improvement, Guangzhou 510642, Guangdong, Peoples R China

5.Univ Chinese Acad Sci, Beijing Genom Inst BGI Educ Ctr, Shenzhen 518083, Guangdong, Peoples R China

关键词： Glycine max; Powdery mildew; Adult-plant resistance; Gene; Fine mapping

期刊名称：CROP JOURNAL （ 影响因子：4.647； 五年影响因子：5.781 ）

ISSN： 2095-5421

年卷期： 2022 年 10 卷 4 期

页码：

收录情况： SCI

摘要： Powdery mildew (PM), caused by the fungus Microsphaera diffusa, causes severe yield losses in soybean [Glycine max (L.) Merr.] under suitable environmental conditions. Identifying resistance genes and developing resistant cultivars may prevent soybean PM damage. In this study, analysis of F-1, F-2, and F8:11 recombinant inbred line (RIL) populations derived from the cross between Zhonghuang 24 (ZH24) and Huaxia 3 (HX3) indicated that adult-plant resistance (APR) to powdery mildew in the soybean cultivar (cv.) ZH24 was controlled by a single dominant locus. A high-density genetic linkage map of the RIL population was used for fine mapping. The APR locus in ZH24 was mapped to a 281-kb genomic region on chromosome 16. Using 283 susceptible plants of another F-2 population, the candidate region was fine -mapped to a 32.8-kb genomic interval flanked by the markers InDel14 and Gm16_428. The interval harbored five genes, including four disease resistance (R)-like genes, according to the Williams 82.a2.v1 reference genome. Quantitative real-time PCR assays of candidate genes revealed that the expression levels of Glyma.16g214300 and Glyma.16g214500 were changed by M. diffusa infection and might be involved in disease defense. Rmd_B13 showed all-stage resistance (ASR) to PM in soybean cv. B13. An allelism test in the F-2 segregating population from the cross of ZH24 x B13 suggested that the APR locus Rmd_ZH24 and the ASR locus Rmd_B13 may be allelic or tightly linked. These results provide a reference marker-assisted selection in breeding programs. (C) 2022 Crop Science Society of China and Institute of Crop Science, CAAS. Production and hosting by Elsevier B.V. on behalf of KeAi Communications Co., Ltd.

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