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High-throughput extraction and automatic purification of alternariol from edible and medicinal herbs based on aptamer-functionalized magnetic nanoparticles

文献类型： 外文期刊

作者： Yang, Ruiqi 1 ; Zhao, Liping 2 ; Wang, Fengchao 3 ; Chen, Jin 3 ; Ma, Xiao 1 ; Luan, Yunxia 2 ; Kong, Weijun 1 ;

作者机构： 1.Chengdu Univ Tradit Chinese Med, Pharm Coll, State Key Lab Southwestern Chinese Med Resources, Chengdu 611137, Peoples R China

2.Beijing Acad Agr & Forestry Sci, Inst Qual Stand & Testing Technol, Beijing, Peoples R China

3.Shanghai Inst Technol, Coll Sci, Shanghai, Peoples R China

4.Capital Med Univ, Sch Tradit Chinese Med, Beijing, Peoples R China

5.BAAFS, Inst Qual Stand & Testing Technol, Beijing 100097, Peoples R China

关键词： alternariol mycotoxin; aptamer; edible herbs; magnetic solid-phase extraction; sample pre-treatmen

期刊名称：JOURNAL OF SEPARATION SCIENCE （ 影响因子：2.8； 五年影响因子：2.7 ）

ISSN： 1615-9306

年卷期： 2024 年 47 卷 5 期

页码：

收录情况： SCI

摘要： Mycotoxin contamination is widespread in plants and herbs, posing serious threats to the consumer and human health. Of them, alternariol (AOH) has attracted great attention as an "emerging" mycotoxin in medicinal herbs. However, a specific and high-throughput extraction method for AOH is currently lacking. Thus, developing an efficient pre-treatment technique for AOH detection is extremely vital. Here, a novel automated magnetic solid-phase extraction method was proposed for the highly efficient extraction of AOH. Combining the aptamer-functionalized magnetic nanoparticles (AMNPs) and the automatic purification instrument, AOH could be extracted in medicinal herbs in high throughput (20 samples) and a short time (30 min). The main parameters affecting extraction were optimized, and the method was finally carried out by incubation AMNPs with 3 mL of sample solution for 10 min, and then desorption in 75% methanol for liquid-phase detection. Under optimal conditions, good reproducibility, stability, and selectivity were realized with an adsorption capacity of 550.84 ng/mg. AOH extraction in three edible herbs showed good resistance to matrix interference with recovery rates from 86% to 111%. In combination with AMNPs and the automatic purification instrument, high-throughput and labor-free extraction of AOH in different complex matrices was achieved, which could be extended in other complex matrices.

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