Microfluidic synthesis of pH-responsive molecularly imprinted silica nanospheres for fluorescence sensing target glycoprotein
文献类型： 外文期刊
作者： Li, Qianjin 1 ; Wang, Meng 1 ; Jin, Yu 1 ; Lu, Yongling 1 ; Xiong, Shuqing 1 ; Wang, Mengdi 1 ; Xu, Jianhong 2 ; Wei, Chenhong 3 ; Li, Jianlin 1 ;
作者机构： 1.Nanjing Normal Univ, Sch Food Sci & Pharmaceut Engn, Nanjing 210023, Peoples R China
2.Jiangsu Acad Agr Sci, Jiangsu Key Lab Food Qual & Safety, State Key Lab Cultivat Base, Minist Sci & Technol, Nanjing 210014, Peoples R China
3.Anhui Costar Biochem Co Ltd, Dangtu 243100, Anhui, Peoples R China
关键词： Molecularly imprinted polymer; Fluorescent sensor; Microfluidic reactor; Phenylboronic acid; Ovalbumin
期刊名称：FOOD CHEMISTRY （ 影响因子：8.8； 五年影响因子：8.6 ）
ISSN： 0308-8146
年卷期： 2023 年 426 卷
页码：
收录情况： SCI
摘要： Here, fluorescent artificial antibodies for sensing ovalbumin in food were synthesized by molecular imprinting technique in a microfluidic reactor. A phenylboronic acid-functionalized silane was employed as the functional monomer to enable the polymer has pH-responsive property. Fluorescent molecularly imprinted polymers (FMIPs) could be produced continuously in a short time. Both fluorescein isothiocyanate (FITC) and rhodamine B isothiocyanate (RB)-based FMIPs can specifically recognize the target ovalbumin, particularly FITC-based FMIP, giving an imprinting factor of 2.5 and cross-reactivity factors of 2.7 (ovotransferrin), 2.8 (& beta;-lactoglobulin) and 3.4 (bovine serum albumin), and was applied for the detection of ovalbumin in milk powder with recovery rates of 93-110%; moreover, the FMIP can be reused at least four times. Such FMIPs have promising future in replacing the fluorophore-labelled antibodies to fabricate fluorescent sensing devices or establish immunoassay methods, which have extra merits of low-cost, high stability and recyclability, easy to carry and store at ambient environments.
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