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The key regulator LcERF056 enhances salt tolerance by modulating reactive oxygen species-related genes in Lotus corniculatus

文献类型： 外文期刊

作者： Wang, Dan 1 ; Sun, Zhanmin 1 ; Hu, Xinxu 3 ; Xiong, Junbo 4 ; Hu, Lizhen 5 ; Xu, Yuandong 6 ; Tang, Yixiong 1 ; Wu, Yanmin 1 ;

作者机构： 1.Chinese Acad Agr Sci, Biotechnol Res Inst, Beijing, Peoples R China

2.China West Normal Univ, Minist Educ, Key Lab Southwest China Wildlife Resources Conser, Nanchong, Peoples R China

3.Hunan Agr Univ, Coll Anim Sci & Technol, Changsha, Peoples R China

4.Hubei Acad Agr Sci, Inst Anim & Vet Sci, Hubei Key Lab Anim Embryo & Mol Breeding, Wuhan, Peoples R China

5.Jiangxi Acad Agr Sci, Inst Anim & Vet Sci, Nanchang, Jiangxi, Peoples R China

6.ChongQing Acad Anim Sci, Chongqing, Peoples R China

7.Huanshan Grp, Qingdao, Peoples R China

关键词： ERF transcription factor; Salt stress; Regulation mechanism; Lotus corniculatus

期刊名称：BMC PLANT BIOLOGY （ 影响因子：5.26； 五年影响因子：5.761 ）

ISSN： 1471-2229

年卷期： 2021 年 21 卷 1 期

页码：

收录情况： SCI

摘要： Background The APETALA2/ethylene response factor (AP2/ERF) family are important regulatory factors involved in plants' response to environmental stimuli. However, their roles in salt tolerance in Lotus corniculatus remain unclear. Results Here, the key salt-responsive transcription factor LcERF056 was cloned and characterised. LcERF056 belonging to the B3-1 (IX) subfamily of ERFs was considerably upregulated by salt treatment. LcERF056-fused GFP was exclusively localised to nuclei. Furthermore, LcERF056- overexpression (OE) transgenic Arabidopsis and L. corniculatus lines exhibited significantly high tolerance to salt treatment compared with wild-type (WT) or RNA interference expression (RNAi) transgenic lines at the phenotypic and physiological levels. Transcriptome analysis of OE, RNAi, and WT lines showed that LcERF056 regulated the downstream genes involved in several metabolic pathways. Chromatin immunoprecipitation-quantitative polymerase chain reaction (ChIP-qPCR) and yeast one-hybrid (Y1H) assay demonstrated that LcERF056 could bind to cis-element GCC box or DRE of reactive oxygen species (ROS)-related genes such as lipid-transfer protein, peroxidase and ribosomal protein. Conclusion Our results suggested that the key regulator LcERF056 plays important roles in salt tolerance in L. corniculatus by modulating ROS-related genes. Therefore, it may be a useful target for engineering salt-tolerant L. corniculatus or other crops.

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